Linked Life Data

8741150

Source:http://linkedlifedata.com/resource/pubmed/id/8741150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-11-7
pubmed:abstractText
Apoptosis is a selective event of physiological cell deletion that plays a crucial role in the development of numerous tissues, including the retina. In this paper we report the occurrence of apoptosis in epiretinal membranes derived from patients with proliferative vitreoretinopathy (PVR). Detection of apoptosis was performed by an in situ DNA-end labeling technique using terminal transferase-mediated deoxyuridine triphosphate (dUTP) incorporation. Apoptotic nuclei exhibiting chromatin condensation and fragmentation were also identified by acridine orange fluorescence. Apoptosis was detected in varying numbers of cells. The typical appearance of apoptotic nuclei, including nuclear chromatin condensation, was detected scattered inhomogeneously throughout the epiretinal membranes, in clusters, or even in single cells. Induction of apoptosis in human retinal pigment epithelial (RPE) cells by daunomycin could be demonstrated by in situ DNA end labeling and by quantitative determination of cytoplasmatic histone-associated DNA fragments using a photometric enzyme immunoassay. Since apoptosis has been shown to be an important factor in the growth control of various untransformed and neoplastic cell populations, the pharmacological induction of apoptosis in epiretinal membranes could result in a new approach toward inhibiting cellular proliferation in PVR.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0941-2921
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Apoptotic cell death in proliferative vitreoretinopathy.
pubmed:affiliation
Abteilung für Netzhaut- und Glaskörperchirurgie der Universität Köln, Germany. peter.esser@uni-koeln.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't