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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
14
|
| pubmed:dateCreated |
1979-9-1
|
| pubmed:abstractText |
Murine erythrocyte ghosts (from DBA/2, CD-1, and B6D2 strains) contain significant amounts of O-acetylated sialyl residues, which are slowly oxidized by periodate. Sialic acids have been purified from murine erythrocyte ghosts and the existence of O-acetylated sialic acids has been confirmed: 1) by assaying with the Warren procedure before and after de-O-acetylation with 0.1 N NaOH for 45 min at 4 degrees C; 2) by thin layer chromatography on cellulose; and 3) by gas-liquid chromatography. Because these sialyl residues are unevenly distributed on the sialoglycoproteins of murine erythrocyte ghosts, the periodic acid-Schiff staining detects only one major sialoglycoprotein. A modification of the periodic acid-Schiff stain method removes these O-acetyl groups after electrophoresis and reveals two additional sialoglycoproteins which bear the majority of the O-acetylated sialyl residues. Rat erythrocyte ghosts have similar residues on one of their two sialoglycoproteins. Ghosts of human, rabbit, and guinea pig erythrocytes do not contain detectable amounts of O-acetylated sialyl residues.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
254
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
6724-31
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:87397-Acetylation,
pubmed-meshheading:87397-Animals,
pubmed-meshheading:87397-Chromatography, Gas,
pubmed-meshheading:87397-Erythrocyte Membrane,
pubmed-meshheading:87397-Erythrocytes,
pubmed-meshheading:87397-Glycopeptides,
pubmed-meshheading:87397-Guinea Pigs,
pubmed-meshheading:87397-Humans,
pubmed-meshheading:87397-Kinetics,
pubmed-meshheading:87397-Membrane Proteins,
pubmed-meshheading:87397-Mice,
pubmed-meshheading:87397-Oxidation-Reduction,
pubmed-meshheading:87397-Periodic Acid,
pubmed-meshheading:87397-Rabbits,
pubmed-meshheading:87397-Rats,
pubmed-meshheading:87397-Sialoglycoproteins,
pubmed-meshheading:87397-Species Specificity,
pubmed-meshheading:87397-Staining and Labeling
|
| pubmed:year |
1979
|
| pubmed:articleTitle |
The sialoglycoproteins of murine erythrocyte ghosts. A modified periodic acid-Schiff stain procedure staining nonsubstituted and O-acetylated sialyl residues on glycopeptides.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
|