Linked Life Data

8739158

Source:http://linkedlifedata.com/resource/pubmed/id/8739158

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-10-17
pubmed:abstractText
We describe sequence similarity and immunologic cross-reactivity between a peptide of the mycobacterial hsp, HSP65, and the myelin protein 2',3' cyclic nucleotide 3' phosphodiesterase (CNP). We demonstrate that immunization with the homologous cross-reactive CNP peptide (hsp-CNP peptide) has significant biological consequences. Rats immunized with hsp-CNP peptide in either complete Freund's adjuvant (CFA) or incomplete Freund's adjuvant (IFA) produce large amounts of peptide-specific antibody. Isotypes of antibodies in animals immunized with peptide in CFA are IgG1 and IgG2a. Isotypes of antibodies in rats immunized with peptide in IFA are predominantly IgG1, with low titers of IgG2a. T cell proliferative responses to HSP65 are present in rats immunized with peptide in CFA. T cell responses to HSP65 initially are absent in rats immunized with peptide in IFA but develop over time. T cell proliferative responses to hsp-CNP peptide were not detected. None of the groups of rats developed clinical or histologic evidence of experimental autoimmune encephalomyelitis (EAE). To induce EAE, rats preimmunized with hsp-CNP peptide were challenged with guinea pig spinal cord (GPSC) emulsified in CFA. Rats preimmunized with peptide in CFA developed severe EAE. Rats preimmunized with hsp-CNP peptide in IFA were protected from EAE, with both a lower incidence and severity of disease. Injecting the murine monoclonal antibody recognizing the shared HSP65 and CNP epitope did not protect against EAE. Our data suggest that a Th2 pattern of immune response to a CNP peptide that itself is non-encephalitogenic protects against EAE. Immune responses to either hsp or myelin proteins cross-reactive with hsp may play an important role in the development of EAE.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0360-4012
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
381-96
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8739158-2',3'-Cyclic-Nucleotide Phosphodiesterases, pubmed-meshheading:8739158-Amino Acid Sequence, pubmed-meshheading:8739158-Animals, pubmed-meshheading:8739158-Antibodies, Monoclonal, pubmed-meshheading:8739158-Antibody Formation, pubmed-meshheading:8739158-Bacterial Proteins, pubmed-meshheading:8739158-Chaperonin 60, pubmed-meshheading:8739158-Chaperonins, pubmed-meshheading:8739158-Encephalomyelitis, Autoimmune, Experimental, pubmed-meshheading:8739158-Epitopes, pubmed-meshheading:8739158-Female, pubmed-meshheading:8739158-Freund's Adjuvant, pubmed-meshheading:8739158-Guinea Pigs, pubmed-meshheading:8739158-Humans, pubmed-meshheading:8739158-Immunity, Cellular, pubmed-meshheading:8739158-Immunoglobulin G, pubmed-meshheading:8739158-Immunoglobulin Isotypes, pubmed-meshheading:8739158-Lymphocyte Activation, pubmed-meshheading:8739158-Mice, pubmed-meshheading:8739158-Molecular Sequence Data, pubmed-meshheading:8739158-Myelin Sheath, pubmed-meshheading:8739158-Rats, pubmed-meshheading:8739158-Rats, Inbred Lew, pubmed-meshheading:8739158-Recombinant Proteins, pubmed-meshheading:8739158-Spinal Cord, pubmed-meshheading:8739158-Time Factors
pubmed:year
1996
pubmed:articleTitle
Heat shock proteins and experimental autoimmune encephalomyelitis (EAE): I. Immunization with a peptide of the myelin protein 2',3' cyclic nucleotide 3' phosphodiesterase that is cross-reactive with a heat shock protein alters the course of EAE.
pubmed:affiliation
Department of Neurology, University of Minnesota, Minneapolis 55455, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't