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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1997-1-28
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pubmed:abstractText |
A sensitive reversed-phase high-performance liquid chromatographic fluorescence method is described for the simultaneous determination of topotecan (I) and the hydrolysed lactone ring-opened product hydroxy acid (II) in plasma and for the determination of I in urine. To 250 microliters of plasma, a 750-microliters volume of cold methanol was added to stabilize the pH-dependent conversion of I into II. In plasma, the lower limit of quantitation (LLQ) for both compounds was 0.10 ng/ml. The between-day variation for I at the LLQ was 7.1% and for II was 5.5%. Prior to injection, urine samples were acidified with orthophosphoric acid and diluted with phosphate-buffered saline (PBS). In urine, the calibration curve for I was linear in the range of 10 to 250 ng/ml and the LLQ was 10 ng/ml. The assay was developed to enable pharmacological analysis of I, in on-going phase I and II studies, in patients with solid tumors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Camptothecin,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxy Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Topoisomerase I Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Topotecan
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1572-6495
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
678
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
309-15
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8738036-Camptothecin,
pubmed-meshheading:8738036-Chromatography, High Pressure Liquid,
pubmed-meshheading:8738036-Drug Stability,
pubmed-meshheading:8738036-Enzyme Inhibitors,
pubmed-meshheading:8738036-Humans,
pubmed-meshheading:8738036-Hydrogen-Ion Concentration,
pubmed-meshheading:8738036-Hydrolysis,
pubmed-meshheading:8738036-Hydroxy Acids,
pubmed-meshheading:8738036-Kinetics,
pubmed-meshheading:8738036-Regression Analysis,
pubmed-meshheading:8738036-Sensitivity and Specificity,
pubmed-meshheading:8738036-Topoisomerase I Inhibitors,
pubmed-meshheading:8738036-Topotecan
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pubmed:year |
1996
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pubmed:articleTitle |
Sensitive high-performance liquid chromatographic fluorescence assay for the quantitation of topotecan (SKF 104864-A) and its lactone ring-opened product (hydroxy acid) in human plasma and urine.
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pubmed:affiliation |
Department of Medical Oncology, Rotterdam Cancer Institute/University Hospital Rotterdam, Netherlands.
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pubmed:publicationType |
Journal Article
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