Linked Life Data

8737491

Source:http://linkedlifedata.com/resource/pubmed/id/8737491

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-9-20
pubmed:abstractText
We inoculated 5 to 7-week-old female C3HeB/FeJ mice with Streptococcus pyogenes strain B514-Sm (type M50) by both an intranasal and intratracheal route and characterized the resulting illness. Following intranasal inoculation, the animals developed signs of illness within 1 to 8 days post-inoculation which correlated with acute, suppurative, bronchopneumonia during histopathologic analysis; however, the relationship of response to dose was non-linear, as seen previously in a small group of mice. Intratracheal inoculations were then performed to increase the quantitative reliability of the model. Following intratracheal inoculation, the animals succumbed to an illness that was indistinguishable from that seen after intranasal inoculation, and the incidence of pneumonia followed a steep dose response curve. The dose at which 50% of the animals exhibited signs of respiratory illness within 72 h was 1.0 x 10(7) colony forming units. All of the animals that appeared ill had lung lesions as determined by gross and histopathologic examination. Bacteraemia followed pneumonia in two-thirds of the intratracheally inoculated animals, indicating that the S. pyogenes had crossed tissue barriers. We hope that this model will be useful in future studies concerning the role of suspected streptococcal virulence factors in the later stages of pathogenesis of invasive S. pyogenes infection.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0882-4010
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
213-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Streptococcus pyogenes infection in mice.
pubmed:affiliation
Department of Microbiology and Immunology, Emory University, Atlanta, GA 30322, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.