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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0014533,
umls-concept:C0014609,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0036536,
umls-concept:C0036537,
umls-concept:C0085862,
umls-concept:C0205111,
umls-concept:C0332120,
umls-concept:C0596019,
umls-concept:C0598850,
umls-concept:C1299583,
umls-concept:C1314939,
umls-concept:C1549571,
umls-concept:C1608386
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pubmed:issue |
3
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pubmed:dateCreated |
1996-11-12
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pubmed:abstractText |
Electrogenic chloride and bicarbonate secretion by cultured rat epididymal epithelia was studied using the short-circuit current (ISC) technique. When incubated in normal solution, 8-(4-chlorophenylthio)-adenosine 3',5'-cyclic monophosphate (cpt-cAMP) caused a rise in the ISC, which was attributable to Cl- and HCO3- secretion. Cl- secretion was found to contribute to the initial transient phase, whereas HCO3- secretion contributed to the sustained phase of the response. HCO3- secretion involves a basolaterally placed Na(+)-H+ exchanger and apical anion channel, most probably the cystic fibrosis transmembrane conductance regulator (CFTR). There is also evidence that an apical electrogenic Na(+)-HCO3- cotransporter is involved in HCO3- exit. CFTR accounted for 70% of HCO3- secretion, while the Na(+)-HCO3- cotransporter accounted for 30%. The possibility that the cotransporter may serve as an alternative pathway for HCO3- secretion in cystic fibrosis is discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/4-Acetamido-4'-isothiocyanatostilben...,
http://linkedlifedata.com/resource/pubmed/chemical/8-((4-chlorophenyl)thio)cyclic-3',5'...,
http://linkedlifedata.com/resource/pubmed/chemical/Acetazolamide,
http://linkedlifedata.com/resource/pubmed/chemical/Amiloride,
http://linkedlifedata.com/resource/pubmed/chemical/Bicarbonates,
http://linkedlifedata.com/resource/pubmed/chemical/Bumetanide,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Hexokinase,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0958-0670
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
515-24
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8737084-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:8737084-4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid,
pubmed-meshheading:8737084-Acetazolamide,
pubmed-meshheading:8737084-Amiloride,
pubmed-meshheading:8737084-Animals,
pubmed-meshheading:8737084-Bicarbonates,
pubmed-meshheading:8737084-Bumetanide,
pubmed-meshheading:8737084-Carrier Proteins,
pubmed-meshheading:8737084-Cells, Cultured,
pubmed-meshheading:8737084-Chlorides,
pubmed-meshheading:8737084-Culture Media,
pubmed-meshheading:8737084-Cyclic AMP,
pubmed-meshheading:8737084-Electrophysiology,
pubmed-meshheading:8737084-Epididymis,
pubmed-meshheading:8737084-Epithelium,
pubmed-meshheading:8737084-Hexokinase,
pubmed-meshheading:8737084-Male,
pubmed-meshheading:8737084-Membrane Potentials,
pubmed-meshheading:8737084-Rats,
pubmed-meshheading:8737084-Rats, Sprague-Dawley,
pubmed-meshheading:8737084-Thionucleotides
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pubmed:year |
1996
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pubmed:articleTitle |
Evidence for independent Cl- and HCO3- secretion and involvement of an apical Na(+)-HCO3- cotransporter in cultured rat epididymal epithelia.
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pubmed:affiliation |
Department of Physiology, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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