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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1996-12-10
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pubmed:abstractText |
We evaluated two immunochromatographic assays (ICAs), Dainascreen HBsAg for detecting human hepatitis hepatitis B surface antigen (HBsAg) and Dainascreen Ausab for detecting human hepatitis B surface antibody (anti-HBs) in human serum. The ICA systems are composed of a comb-shaped device that contains nitrocellulose strips on which complexes of HBsAg and anti-HBs can be visualized. The results can be read within 15 min of incubation. The limit of detection for HBsAg was 3.1 ng/ml and that for anti-HBs was 42 mIU/ml. Results of HBsAg detection agreed completely with those of conventional enzyme immunoassays (EIAs) and showed a 100% sensitivity (158 of 158 samples) and a 100% specificity (304 of 304 samples). The Dainascreen Ausab detected 184 of the 199 EIA-positive samples (sensitivity, 92.5%) and yielded 6 positive results among the 281 EIA-negative samples (specificity, 97.9%). The ICA systems are rapid and sensitive methods for detecting HBsAg and anti-HBs. They are low-cost systems that need no complex instrumentation for analysis and can be recommended for routine use in clinical microbiology laboratories.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0095-1137
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1420-2
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8735091-Chromatography,
pubmed-meshheading:8735091-Collodion,
pubmed-meshheading:8735091-Diagnostic Errors,
pubmed-meshheading:8735091-Evaluation Studies as Topic,
pubmed-meshheading:8735091-Hepatitis B,
pubmed-meshheading:8735091-Hepatitis B Antibodies,
pubmed-meshheading:8735091-Hepatitis B Surface Antigens,
pubmed-meshheading:8735091-Humans,
pubmed-meshheading:8735091-Immunoassay,
pubmed-meshheading:8735091-Immunoenzyme Techniques,
pubmed-meshheading:8735091-Sensitivity and Specificity,
pubmed-meshheading:8735091-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Evaluation of immunochromatographic assay systems for rapid detection of hepatitis B surface antigen and antibody, Dainascreen HBsAg and Dainascreen Ausab.
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pubmed:affiliation |
Department of Clinical Laboratory Medicine, Nagoya University Hospital, Japan.
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pubmed:publicationType |
Journal Article
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