Linked Life Data

8720693

Source:http://linkedlifedata.com/resource/pubmed/id/8720693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-10-31
pubmed:abstractText
The mechanism of action of MD1, a new biotechnological radiotherapeutic agent of plant origin, on smooth muscle contraction was investigated. Contraction induced by MD1 consists of an initial dose-dependent transient phasic response (PR) (with ED50 = 0.11 +/- 0.02%, n = 4, the time of rise 15 s, onset of decay about 30 s) followed by a tonic contraction (TC) (at 10% MD1 only) with a maximum in 20 min (0.78 +/- 0.07 x the maximum contraction induced by 110 mmol/l KCl, n = 8, (TC/PR) = 0.94 +/- 0.11, n = 5). If the vessel was washed out during this maximum, the maximum tension was maintained for up to 2 h ("long-lasting" tension) and was abolished after perfusion with Ca(2+)-free EGTA solution (E) or H7 (0.05 mmol/l) solution. With solution E being applied 30 min before 10% MD1-induced contraction, TC was reduced by 92 +/- 3% (n = 4) in contrast to PR being reduced by 48 +/- 5% (n = 5). During TC, calcium appeared to penetrate into cells through receptor operated channels, since tension neither depended on verapamil (0.05 mmol/l) nor cobalt (up to 10 mmol/l). In solution H7 (with 10 min pretreatment), PR and TC were almost completely inhibited. It is proposed that MD1 activates the C-kinase branch of the calcium messenger system.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0231-5882
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
293-303
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Mechanisms of vasoconstriction induced in frog vascular smooth muscle by MD1, a new biotechnological agent.
pubmed:affiliation
Sechenov Institute of Evolutionary Physiology and Biochemistry, Academy of Sciences of Russia, St. Petersburg. Sobol@IEF.SPB.SU
pubmed:publicationType
Journal Article, In Vitro