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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-9-23
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pubmed:abstractText |
The cytokine IL-1 beta has previously been demonstrated to induce the expression of the stress genes iNOS, hsp70, heme oxygenase and Mn-SOD in rat pancreatic islets in vitro. The aim of this study was to determine whether the IL-1 beta-induced effects are specific for the insulin producing beta-cell, or whether other islet cells, such as the glucagon-producing alpha-cell, respond to IL-1 beta addition. Purified rat alpha- and beta-cell suspensions were obtained by fluorescence-activated cell sorting and incubated with or without IL-1 beta (25 U/ml) for 24 h. The alpha- and beta-cell contents of hsp70, heme oxygenase and Mn-SOD and medium nitrite levels were determined. It was found that IL-1 beta exposure induced the production of nitric oxide in beta-cells, but not in alpha-cells. Moreover, the expression of hsp70, heme oxygenase and Mn-SOD was also induced in beta-cells, but not in alpha-cells. There were no detectable levels of hsp70 in alpha-cells. It is concluded that the stress gene response following IL-1 beta exposure is markedly different in alpha- and beta-cells. This finding may be of importance for the understanding of the autoimmune destruction of beta-cells in insulin-dependent diabetes mellitus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0165-2478
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
48
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
145-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8719114-Animals,
pubmed-meshheading:8719114-Cell Separation,
pubmed-meshheading:8719114-Enzyme Induction,
pubmed-meshheading:8719114-Flow Cytometry,
pubmed-meshheading:8719114-HSP70 Heat-Shock Proteins,
pubmed-meshheading:8719114-Heme Oxygenase (Decyclizing),
pubmed-meshheading:8719114-Immunoblotting,
pubmed-meshheading:8719114-Interleukin-1,
pubmed-meshheading:8719114-Islets of Langerhans,
pubmed-meshheading:8719114-Male,
pubmed-meshheading:8719114-Rats,
pubmed-meshheading:8719114-Rats, Inbred Lew,
pubmed-meshheading:8719114-Superoxide Dismutase
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pubmed:year |
1995
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pubmed:articleTitle |
Interleukin-1 beta induces the expression of hsp70, heme oxygenase and Mn-SOD in FACS-purified rat islet beta-cells, but not in alpha-cells.
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pubmed:affiliation |
Department of Medical Cell Biology, Uppsala University, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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