Linked Life Data

8714763

Source:http://linkedlifedata.com/resource/pubmed/id/8714763

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-10-2
pubmed:abstractText
In order to develop a simple and feasible approach to achieve high frequency plant regeneration for protoplast isolation and transformation experiments, a method was elaborated by using a new type of explant (petiole segments) for the four Populus nigra genotypes. Callus initiation from the petioles took place on N6 medium containing 2,4-D (0.1-1 mg/l). The highest rate of callus initiation (100%) was achieved when the basic medium was supplemented with 0.5 mg/l of 2,4-D, in all tested genotypes. For shoot regeneration, calli were transferred to MS and WPM medium supplemented with BA (1.0-2.5 mg/l) and NAA (0.2 mg/l). Multiple shoot regeneration was observed in each shoot induction medium. The highest rate of shoot regeneration (6.83 shoot/callus) was observed on MS medium containing 2.5 mg/l BA and 0.2 mg/l NAA. The results showed highly significant differences between the media. There was no significant difference between the genotypes and genotype x medium interaction.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0236-5383
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
51-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
High efficiency callus induction and plant regeneration in petiole culture of four poplar genotypes.
pubmed:affiliation
Gödöllõ University of Agricultural Sciences, Department of Genetics and Plant Breeding, Hungary.
pubmed:publicationType
Journal Article, Comparative Study