Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-10-11
|
| pubmed:abstractText |
A one-step sandwich enzyme immunoassay (EIA) system for human matrix metalloproteinase 8 (MMP-8, neutrophil collagenase, EC 3.4.24.7) has been established with a pair of monoclonal antibodies prepared against the zymogen of MMP-8 purified from human neutrophils. MMP-8 in samples simultaneously reacted with both solid-phase and peroxidase-labeled antibodies. Sensitivity of this EIA system was 0.34 micrograms/l (5.7 pg/assay) and linearity was obtained between 0.5 and 500 micrograms/l (8.3-8300 pg/assay). The EIA system recognized both precursor and active forms of MMP-8 but not MMP-8 complexed with tissue inhibitors of metalloproteinases. There was no difference in the MMP-8 levels between the plasma samples from patients with rheumatoid arthritis or osteoarthritis and those from healthy subjects (median 6.2 micrograms/l, range 1.5-28 micrograms/l). However, the level in synovial fluids from patients with rheumatoid arthritis (median 345 micrograms/l, range 84-2860 micrograms/l) was shown to be higher than that from osteoarthritic patients. MMP-8 levels in human whole saliva from patients with periodontal diseases (median 282 micrograms/l, range 0-1420 micrograms/l) were also significantly higher than those from clinically healthy subjects (median 25 micrograms/l, range 0-100 micrograms/l). Immunoreactivity analyses showed that MMP-8 species in normal human plasma exists as a precursor but not as a complex form with tissue inhibitor of metalloproteinases (TIMP)-1 or TIMP-2.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0009-8981
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
31
|
| pubmed:volume |
244
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
129-43
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8714431-Animals,
pubmed-meshheading:8714431-Antibodies, Monoclonal,
pubmed-meshheading:8714431-Antibody Specificity,
pubmed-meshheading:8714431-Arthritis, Rheumatoid,
pubmed-meshheading:8714431-Biological Markers,
pubmed-meshheading:8714431-Cell Line,
pubmed-meshheading:8714431-Chromatography, Affinity,
pubmed-meshheading:8714431-Chromatography, Gel,
pubmed-meshheading:8714431-Collagenases,
pubmed-meshheading:8714431-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8714431-Female,
pubmed-meshheading:8714431-Humans,
pubmed-meshheading:8714431-Immunoenzyme Techniques,
pubmed-meshheading:8714431-Immunohistochemistry,
pubmed-meshheading:8714431-Matrix Metalloproteinase 8,
pubmed-meshheading:8714431-Mice,
pubmed-meshheading:8714431-Mice, Inbred BALB C,
pubmed-meshheading:8714431-Molecular Weight,
pubmed-meshheading:8714431-Neutrophils,
pubmed-meshheading:8714431-Osteoarthritis,
pubmed-meshheading:8714431-Placenta,
pubmed-meshheading:8714431-Pregnancy,
pubmed-meshheading:8714431-Saliva,
pubmed-meshheading:8714431-Statistics, Nonparametric,
pubmed-meshheading:8714431-Stomach Neoplasms,
pubmed-meshheading:8714431-Synovial Fluid,
pubmed-meshheading:8714431-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A one-step sandwich enzyme immunoassay for human matrix metalloproteinase 8 (neutrophil collagenase) using monoclonal antibodies.
|
| pubmed:affiliation |
Biopharmaceutical Department, Fuji Chemical Industries, Ltd., Toyama, Japan.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|