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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
1996-9-12
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pubmed:abstractText |
The crystal structure of the sigma class glutathione transferase from squid digestive gland in complex with S-(3-iodobenzyl)glutathione reveals a third binding site for the glutathione conjugate besides the two in the active sites of the dimer. The additional binding site is near the crystallographic two-fold axis between the two alpha 4-turn-alpha 5 motifs. The principal binding interactions with the conjugate include specific electrostatic interactions between the peptide and the two subunits and a hydrophobic cavity found across the two-fold axis that accommodates the 3-iodobenzyl group. Thus, two identical, symmetry-related but mutually exclusive binding modes for the third conjugate are observed. The hydrophobic pocket is about 14 A from the hydroxyl group of Tyr-7 in the active site. This site is a potential transport binding site for hydrophobic molecules or their glutathione conjugates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-1420139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-1522586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-1782341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-1848757,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-2025413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-2065650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-2774553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-3068032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-3097327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-3864155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-4944188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7538846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7723011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7727393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7774571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7817866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-7853399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8142473,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8143720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8145243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8182750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8241147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8331657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710848-8505281
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8208-13
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8710848-Animals,
pubmed-meshheading:8710848-Binding Sites,
pubmed-meshheading:8710848-Crystallography, X-Ray,
pubmed-meshheading:8710848-Decapodiformes,
pubmed-meshheading:8710848-Glutathione Transferase,
pubmed-meshheading:8710848-Helix-Turn-Helix Motifs,
pubmed-meshheading:8710848-Macromolecular Substances,
pubmed-meshheading:8710848-Models, Molecular,
pubmed-meshheading:8710848-Recombinant Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Location of a potential transport binding site in a sigma class glutathione transferase by x-ray crystallography.
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pubmed:affiliation |
Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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