|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
1996-9-9
|
|
pubmed:abstractText |
The CSRE (carbon source-responsive element) is a sequence motif responsible for the transcriptional activation of gluconeogenic structural genes in Saccharomyces cerevisiae. We have isolated a regulatory gene, DIL1 (derepression of isocitrate lyase, = CAT8), which is specifically required for derepression of CSRE-dependent genes. Expression of CAT8 is carbon source regulated and requires a functional Cat1p (Snf1p) protein kinase. The derepression defect of CAT8 in a cat1 mutant could be suppressed by a mutant Mig1p repressor protein. Derepression of CAT8 also requires a functional HAP2 gene, suggesting a regulatory connection between respiratory and gluconeogenic genes. Carbon source-dependent protein-CSRE complexes detected in a gel retardation analysis with wild-type extracts were absent in cat8 mutant extracts. However, similar experiments with an epitope-tagged CAT8 gene product in the presence of tag-specific antibodies gave evidence against a direct binding of Cat8p to the CSRE. A constitutively expressed GAL4-CAT8 fusion gene revealed a carbon source-dependent transcriptional activation of a UAS(GAL)-containing reporter gene. Activation mediated by Cat8p was no longer detectable in a cat1 mutant. Thus, biosynthetic control of CAT8 as well as transcriptional activation by Cat8p requires a functional Cat1p protein kinase. A model proposing CAT8 as a specific activator of a transcription factor(s) binding to the CSRE is discussed.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1330335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1459453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1471261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1511877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1597176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1729616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-1915298,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-194140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2002006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2167835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2481228,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2648133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2823078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-2832731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-3026915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-3076186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-3319781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-3526554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-6294669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-6387704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-6392017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-6993859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7039847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7607310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7642141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7759539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7854322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7862526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7891685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7900189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7913470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-7945996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8056322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8114729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8196607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8224185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8299163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8319292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8346441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8406042,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8467796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8508802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8557031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710504-8622676
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2331-7
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8710504-Amino Acid Sequence,
pubmed-meshheading:8710504-Base Sequence,
pubmed-meshheading:8710504-Carbon,
pubmed-meshheading:8710504-DNA, Fungal,
pubmed-meshheading:8710504-Fungal Proteins,
pubmed-meshheading:8710504-Gene Dosage,
pubmed-meshheading:8710504-Gene Expression Regulation, Fungal,
pubmed-meshheading:8710504-Gluconeogenesis,
pubmed-meshheading:8710504-Molecular Sequence Data,
pubmed-meshheading:8710504-Mutation,
pubmed-meshheading:8710504-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8710504-Saccharomyces cerevisiae,
pubmed-meshheading:8710504-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:8710504-Trans-Activators,
pubmed-meshheading:8710504-Transcriptional Activation
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Dual influence of the yeast Cat1p (Snf1p) protein kinase on carbon source-dependent transcriptional activation of gluconeogenic genes by the regulatory gene CAT8.
|
|
pubmed:affiliation |
Institut für Mikrobiologie, Biochemie und Genetik, Lehrstuhl Biochemie, Universität Erlangen/Nürnberg, Erlangen, Germany.
|
|
pubmed:publicationType |
Journal Article
|
