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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1996-9-9
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pubmed:abstractText |
Constrained RNA libraries of limited sequence complexity were constructed and used to select RNA molecules binding to the antigen binding site of an anti-ferritin antibody. The sequences required as primer-binding sites for the selection cycle were designed to form a predictable secondary structure, which greatly facilitated the characterisation of the secondary structures of the selected RNAs. RNA-antibody interactions were studied by real-time interaction analysis to study the dynamic aspects of binding and by circular dichroism spectroscopy to search for conformational changes upon binding. The selected RNAs were analysed with a binding site sequestering assay and were shown to compete with ferritin for binding to the antigen-binding site. The experiments described here indicate that the introduction of strong structural constraints does not have to interfere with the ability to select tightly and specifically binding RNA-molecules.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-11539282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-11539574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1379730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1384035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1621097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1690861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1697402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1717938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1741036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-1899841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-2125482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-2200121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-2578319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-2656256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-3089283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7476997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7505429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7510417,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7510636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7521014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7534420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7534830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7545070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7574500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7578958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7668379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7685301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7688943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7723823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7969422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-7973630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-8078068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-8160266,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-8421526,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8710489-8846773
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2220-7
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8710489-Antibodies,
pubmed-meshheading:8710489-Base Sequence,
pubmed-meshheading:8710489-Binding, Competitive,
pubmed-meshheading:8710489-Binding Sites, Antibody,
pubmed-meshheading:8710489-DNA Primers,
pubmed-meshheading:8710489-Ferritins,
pubmed-meshheading:8710489-Gene Library,
pubmed-meshheading:8710489-Kinetics,
pubmed-meshheading:8710489-Molecular Sequence Data,
pubmed-meshheading:8710489-Nucleic Acid Conformation,
pubmed-meshheading:8710489-RNA,
pubmed-meshheading:8710489-Structure-Activity Relationship
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pubmed:year |
1996
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pubmed:articleTitle |
Characterisation of antibody-binding RNAs selected from structurally constrained libraries.
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pubmed:affiliation |
Istituto di Ricerche di Biologia Molecolare, Roma, Italy.
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pubmed:publicationType |
Journal Article
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