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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1996-9-9
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pubmed:abstractText |
Hematopoietic progenitor cells can be classified as plastic- and stroma-adherent (P+S+), stroma-adherent (P-S+) and non-adherent (P-S-). Both P+S+ and P-S+ populations are detected in delta (delta) culture systems where they produce non-adherent (P-S-) granulocyte-macrophage colony-forming cells (CFU-GM) and erythroid burst-forming units (BFU-E). Here we demonstrate that the plastic-adherent progenitor cells (P delta cells) comprise 5-10 percent of the CD34+, population in adult human marrow. Moreover, they do not express CD3 or CD22 and 88 percent of them are CD38-, 88 percent are CD33- and 74 percent are HLA-DR-. Production of CFU-GM by purified plastic-adherent CD34+, adherent cells was 60 percent of the number produced by recombined CD34+, and CD34- fractions. We have shown also that the plastic-adherent P+S+ cells are the precursors of the stroma-adherent P-S+ cells (S delta cells), day 21 cobblestone-area forming cells (CAFC) and cells capable of sustained hematopoiesis in a modified long-term bone marrow culture system. These observations support the primitive nature of P delta cells and establish a phenotypic sequence of plastic and stroma adherence through stroma adherence to non-adherence in hematopoietic cell development. To further investigate the relationship between P delta cells, S delta cells and long-term culture-initiating cells (LTCIC), we cultured whole mononuclear cell tractions and plastic-adherent cell-depleted mononuclear cell fractions in long-term culture and in the S delta assay. The results indicated the P delta cells were inhibited in the presence of stromal cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CD33 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/N-Glycosyl Hydrolases
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0887-6924
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1347-53
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8709641-ADP-ribosyl Cyclase,
pubmed-meshheading:8709641-Adult,
pubmed-meshheading:8709641-Antigens, CD,
pubmed-meshheading:8709641-Antigens, CD3,
pubmed-meshheading:8709641-Antigens, CD34,
pubmed-meshheading:8709641-Antigens, CD38,
pubmed-meshheading:8709641-Antigens, Differentiation,
pubmed-meshheading:8709641-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:8709641-Bone Marrow Cells,
pubmed-meshheading:8709641-Cell Adhesion,
pubmed-meshheading:8709641-Cells, Cultured,
pubmed-meshheading:8709641-Colony-Forming Units Assay,
pubmed-meshheading:8709641-Culture Techniques,
pubmed-meshheading:8709641-HLA-DR Antigens,
pubmed-meshheading:8709641-Hematopoiesis,
pubmed-meshheading:8709641-Hematopoietic Stem Cells,
pubmed-meshheading:8709641-Humans,
pubmed-meshheading:8709641-Immunophenotyping,
pubmed-meshheading:8709641-Membrane Glycoproteins,
pubmed-meshheading:8709641-N-Glycosyl Hydrolases,
pubmed-meshheading:8709641-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Phenotype and progeny of primitive adherent human hematopoietic progenitors.
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pubmed:affiliation |
LRF Centre for Adult Leukaemia, Royal Postgraduate Medical School, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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