Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-9-12
|
| pubmed:abstractText |
The liver plays a central role in the IGF-I axis producing the majority of circulating hormone and some of its binding proteins (IGFBPs). Cirrhosis of the liver is characterised by changes in IGF-I and IGFBPs associated with liver fibrosis and regeneration. We have studied steady state levels of mRNA for the genes in the IGF-I axis in normal and cirrhotic human liver, localised the most highly expressed gene, IGFBP-1, and measured circulating IGFBP-3 by radioimmunoassay (RIA), IGFBP-2 and IGFBP-3 by Western ligand blot (WLB), and protease activity for IGFBP-3 in cirrhotic patients. Messenger RNA for IGF-I, IGFBP-1, IGFBP-2, and IGFBP-3 was detectable by Northern blotting in normal and cirrhotic liver although there was considerable variation in expression. IGFBP-2 and IGFBP-3 tended to be more highly expressed in cirrhotic liver and IGFBP-1 was more highly expressed in normal liver, although there were no significant differences. In normal liver, in situ hybridisation localised IGFBP-1 to hepatocytes. In cirrhotic liver the regenerating nodules showed expression of IGFBP-1 while there was none in fibrotic tissue. Circulating IGFBP-3 levels were low as measured by RIA and WLB but protease activity was only found in one patient. IGFBP-2 levels, assessed by WLB, were similar to the normal serum pool. Our data show that key mRNAs involved in the IGF-I axis continue to be expressed in cirrhotic liver despite end stage liver disease. The low levels of IGFBP-3 do not appear to be due to reduced gene transcription or increased protease activity.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin-Like Growth Factor I,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-0795
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
149
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
209-16
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8708531-Adolescent,
pubmed-meshheading:8708531-Adult,
pubmed-meshheading:8708531-Blotting, Northern,
pubmed-meshheading:8708531-Blotting, Western,
pubmed-meshheading:8708531-Child,
pubmed-meshheading:8708531-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8708531-Female,
pubmed-meshheading:8708531-Gene Expression,
pubmed-meshheading:8708531-Humans,
pubmed-meshheading:8708531-In Situ Hybridization,
pubmed-meshheading:8708531-Insulin-Like Growth Factor Binding Protein 1,
pubmed-meshheading:8708531-Insulin-Like Growth Factor Binding Protein 2,
pubmed-meshheading:8708531-Insulin-Like Growth Factor Binding Protein 3,
pubmed-meshheading:8708531-Insulin-Like Growth Factor Binding Proteins,
pubmed-meshheading:8708531-Insulin-Like Growth Factor I,
pubmed-meshheading:8708531-Liver Cirrhosis,
pubmed-meshheading:8708531-Male,
pubmed-meshheading:8708531-Middle Aged,
pubmed-meshheading:8708531-Peptide Hydrolases,
pubmed-meshheading:8708531-RNA, Messenger,
pubmed-meshheading:8708531-Regeneration
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Expression of IGF-I and IGF-binding protein genes in cirrhotic liver.
|
| pubmed:affiliation |
Department of Medicine, University of Sheffield, UK.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|