Linked Life Data

8707262

Source:http://linkedlifedata.com/resource/pubmed/id/8707262

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-9-10
pubmed:abstractText
On six human hepatocellular carcinoma (HCC) cell lines (KIM-1, KYN-1, KYN-2, KYN-3, HAK-1A, and HAK- 1B), we examined expressions and functions of the proteins and messenger RNAs (mRNAs) of basic fibroblast growth factor (bFGF) and its receptor, i.e., fibroblast growth factor receptor-1 (FGFR-1), as well as mRNA expressions of FGFR-2 approximately 4. All six cell lines expressed the proteins and mRNAs of bFGF and FGFR-1, and at least one of FGFR-2 approximately 4 mRNAs. Two of the six cell lines (KYN-1 and KYN-3) presented significant release of bFGF in culture supernatant, while the release in the remaining four cell lines was quite small. Addition of anti-bFGF neutralizing antibody (1, 10, or 20 microg/mL) to culture medium resulted in marked suppression of cell proliferation in all cell lines except HAK-1A. On the other hand, addition of exogenous bFGF (0.1, 1, or 5 ng/mL) to culture medium stimulated cell proliferation except in KIM-1 and KYN-2. When KIM-1 was transplanted to nude mice and anti-bFGF antibody was injected subcutaneously to a space surrounding the developed tumor, tumor proliferation was significantly suppressed in nude mice that received anti-bFGF antibody than in control mice, but there were no histological differences between the groups, including blood space formation in the stroma. In conclusion, hepatocellular carcinoma (HCC) cells may possess a proliferation mechanism regulated by an autocrine mechanism, a paracrine mechanism, or both, which are mediated by bFGF/FGFR.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0270-9139
pubmed:author
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
198-205
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8707262-Animals, pubmed-meshheading:8707262-Antibodies, pubmed-meshheading:8707262-Carcinoma, Hepatocellular, pubmed-meshheading:8707262-Cell Division, pubmed-meshheading:8707262-Cell Line, pubmed-meshheading:8707262-Fibroblast Growth Factor 2, pubmed-meshheading:8707262-Gene Expression, pubmed-meshheading:8707262-Liver Neoplasms, pubmed-meshheading:8707262-Mice, pubmed-meshheading:8707262-Mice, Nude, pubmed-meshheading:8707262-RNA, Messenger, pubmed-meshheading:8707262-Receptor, Fibroblast Growth Factor, Type 1, pubmed-meshheading:8707262-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:8707262-Receptors, Fibroblast Growth Factor, pubmed-meshheading:8707262-Transcription, Genetic, pubmed-meshheading:8707262-Transplantation, Heterologous, pubmed-meshheading:8707262-Tumor Cells, Cultured
pubmed:year
1996
pubmed:articleTitle
Expressions of basic fibroblast growth factor and its receptors and their relationship to proliferation of human hepatocellular carcinoma cell lines.
pubmed:affiliation
First Department of Pathology, Kurume University School of Medicine, Kurume 830, Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't