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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1996-9-10
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pubmed:abstractText |
In this study, the relative abundance of splicing variants of Oreochromis non-NMDA subtype glutamate receptors was studied by quantitative reverse-transcriptase PCR (RT-PCR). The relative expression level between the flip and flop transcripts of fGluR2 alpha determined by quantitative RT-PCR is apparently much higher than that estimated by sequence analysis of the cloned RT-PCR products. Control studies were performed to demonstrate the accuracy of the application of quantitative RT-PCR analysis in studying the relative abundance between the flip and flop transcripts of glutamate receptors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
22
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pubmed:volume |
390
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-60
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8706849-Alternative Splicing,
pubmed-meshheading:8706849-Animals,
pubmed-meshheading:8706849-Base Sequence,
pubmed-meshheading:8706849-Brain,
pubmed-meshheading:8706849-DNA, Complementary,
pubmed-meshheading:8706849-DNA Primers,
pubmed-meshheading:8706849-Exons,
pubmed-meshheading:8706849-Genetic Variation,
pubmed-meshheading:8706849-Molecular Sequence Data,
pubmed-meshheading:8706849-Polymerase Chain Reaction,
pubmed-meshheading:8706849-RNA,
pubmed-meshheading:8706849-Receptors, Glutamate,
pubmed-meshheading:8706849-Tilapia
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pubmed:year |
1996
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pubmed:articleTitle |
Determination of relative abundance of splicing variants of Oreochromis glutamate receptors by quantitative reverse-transcriptase PCR.
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pubmed:affiliation |
Institute of Radiation Biology, National Tsing-Hua University, Hsinchu, Taiwan, Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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