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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-9-6
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pubmed:abstractText |
The three-dimensional structure of Escherichia coli 3-methyladenine DNA glycosylase II, which removes numerous alkylated bases from DNA, was solved at 2.3 A resolution. The enzyme consists of three domains: one alpha + beta fold domain with a similarity to one-half of the eukaryotic TATA box-binding protein, and two all alpha-helical domains similar to those of Escherichia coli endonuclease III with combined N-glycosylase/abasic lyase activity. Mutagenesis and model-building studies suggest that the active site is located in a cleft between the two helical domains and that the enzyme flips the target base out of the DNA duplex into the active-site cleft. The structure of the active site implies broad substrate specificity and simple N-glycosylase activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:FujiiSS,
pubmed-author:FujiiYY,
pubmed-author:IharaKK,
pubmed-author:KatoMM,
pubmed-author:MatsushimaNN,
pubmed-author:NakabeppuYY,
pubmed-author:NakashimaYY,
pubmed-author:OdawaraKK,
pubmed-author:SekiguchiMM,
pubmed-author:TokunoYY,
pubmed-author:TomitaKK,
pubmed-author:YamagataYY,
pubmed-author:YasumuraKK
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
86
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
311-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8706135-Amino Acid Sequence,
pubmed-meshheading:8706135-Binding Sites,
pubmed-meshheading:8706135-Crystallography,
pubmed-meshheading:8706135-DNA, Bacterial,
pubmed-meshheading:8706135-DNA Glycosylases,
pubmed-meshheading:8706135-DNA Repair,
pubmed-meshheading:8706135-DNA-Binding Proteins,
pubmed-meshheading:8706135-Escherichia coli,
pubmed-meshheading:8706135-Molecular Sequence Data,
pubmed-meshheading:8706135-N-Glycosyl Hydrolases,
pubmed-meshheading:8706135-Protein Conformation,
pubmed-meshheading:8706135-Protein Structure, Tertiary,
pubmed-meshheading:8706135-TATA Box
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pubmed:year |
1996
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pubmed:articleTitle |
Three-dimensional structure of a DNA repair enzyme, 3-methyladenine DNA glycosylase II, from Escherichia coli.
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pubmed:affiliation |
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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