Linked Life Data

8704126

Source:http://linkedlifedata.com/resource/pubmed/id/8704126

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-9-10
pubmed:databankReference
pubmed:abstractText
Flavanone 3-hydroxylase is an enzyme acting in the central part of the flavonoid biosynthesis pathway. It is generally encoded by a single gene and seems to have a key position for the regulation in this pathway. These two features make a single f3h promoter-gus fusion a suitable tool to study both the f3h expression and the regulation of this pathway. We present here the spatial and temporal analysis of the expression of an alfalfa flavanone 3-hydroxylase (f3h) promoter-gus fusion introduced into Nicotiana benthamiana. The Medicago sativa (alfalfa) f3h promoter directed gus expression in flowers, stems, leaves and roots. In flowers, GUS activity was observed in pollen grains, in ovules, in ovary placenta and in the epidermis, medullary parenchyma, trichomes and second cortical cellular layer surrounding the vascular bundles of the peduncle. In stems, GUS activity was detected at the same places as in the peduncle except for the medullary parenchyma. In roots, we found GUS staining in root hairs, epidermis and in the vascular bundles of the elongated zone. Finally, in leaves, the f3h promoter expressed essentially in the stalk cells of the multicellular trichomes. The expression pattern of the f3h-gus fusion was correlated to the presence of flavonoids in situ. These data indicate that this construct can be very useful to study factors controlling the production of flavonoids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1153-68
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8704126-Base Sequence, pubmed-meshheading:8704126-DNA, Plant, pubmed-meshheading:8704126-Flavonoids, pubmed-meshheading:8704126-Gene Expression Regulation, Enzymologic, pubmed-meshheading:8704126-Gene Expression Regulation, Plant, pubmed-meshheading:8704126-Glucuronidase, pubmed-meshheading:8704126-In Situ Hybridization, pubmed-meshheading:8704126-Medicago sativa, pubmed-meshheading:8704126-Mixed Function Oxygenases, pubmed-meshheading:8704126-Molecular Sequence Data, pubmed-meshheading:8704126-Plants, Genetically Modified, pubmed-meshheading:8704126-Plants, Toxic, pubmed-meshheading:8704126-Polymerase Chain Reaction, pubmed-meshheading:8704126-Promoter Regions, Genetic, pubmed-meshheading:8704126-RNA, Messenger, pubmed-meshheading:8704126-Spectrometry, Fluorescence, pubmed-meshheading:8704126-Tobacco
pubmed:year
1996
pubmed:articleTitle
The expression pattern of alfalfa flavanone 3-hydroxylase promoter-gus fusion in Nicotiana benthamiana correlates with the presence of flavonoids detected in situ.
pubmed:affiliation
Institut des Sciences Végétales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't