Linked Life Data

8703584

Source:http://linkedlifedata.com/resource/pubmed/id/8703584

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-9-12
pubmed:abstractText
We investigated the effect of small shifts in extracellular pH on the resorptive activity of rat osteoclasts in vitro. Osteoclast-containing mixed bone cell populations disaggregated from neonatal rat long bones were cultured for 26 h at low density on bovine bone disks in HCO-3/CO2-buffered medium modified by the addition of small amounts of protons as HCl (2.5-15.0 mEq/L) or hydroxyl ions as NaOH (2.5-10.0 mEq/L). Little or no bone resorption occurred when ambient pH was above about 7.30; at pH 7.00, osteoclasts exhibited near-maximal activity. The greatest changes were associated with a pH difference of as little as 0.10 unit, between pH 7.25 and pH 7.15. Over this narrow range, the average number of resorption pits formed on each bone wafer increased sixfold. The steep sigmoidal pH response curve indicates that very slight alterations in ambient hydrogen ion concentration can effectively "switch on" or "switch off" rat osteoclasts in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
8756-3282
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
277-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Modulation of the resorptive activity of rat osteoclasts by small changes in extracellular pH near the physiological range.
pubmed:affiliation
Department of Anatomy and Developmental Biology, University College London, UK.
pubmed:publicationType
Journal Article, Comparative Study