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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
1996-10-11
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pubmed:databankReference |
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pubmed:abstractText |
The cyclosporin A (CsA)/FK506-sensitive nuclear factor of activated T cells (NFAT) plays a key role in the inducible expression of cytokine genes in T cells. Although NFAT has been recently shown to be inducible in several non-T immune cells, the NFAT gene family members characterized to date have been isolated only from T cells. To further characterize NFAT function in human B cells and to demonstrate cytokine gene specificity of NFAT proteins, we report here the isolation and characterization of a cDNA clone from the Raji B cell line. The cDNA clone encodes a new isoform, NFATc.beta, of the NFAT gene family member NFATc (designated here NFATc.alpha). The amino acid sequence of NFATc.beta differs from that of NFATc. alpha in the first NH2-terminal 29 residues and contains an additional region of 142 residues at the COOH terminus. Northern analysis using a probe encompassing a common region of both isoforms showed two mRNA species of 2.7 and 4.5 kilobase pairs, while an NFATc.beta-specific probe detected only the 4.5-kilobase pair mRNA which was preferentially expressed in the spleen. Transient expression of NFATc.beta was capable of activating an interleukin-2 NFAT-driven reporter gene in stimulated Jurkat cells in a CsA-sensitive manner. However, NFATc.beta neither bound to the kappa3 element (an NFAT-binding site) in the tumor necrosis factor-alpha promoter nor activated the tumor necrosis factor-alpha promoter in cotransfection assays. These data suggest that different members or isoforms of NFAT gene family may regulate inducible expression of different cytokine genes.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor AP-1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20914-21
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8702849-Amino Acid Sequence,
pubmed-meshheading:8702849-B-Lymphocytes,
pubmed-meshheading:8702849-Base Sequence,
pubmed-meshheading:8702849-Burkitt Lymphoma,
pubmed-meshheading:8702849-DNA, Complementary,
pubmed-meshheading:8702849-DNA-Binding Proteins,
pubmed-meshheading:8702849-Gene Expression Regulation,
pubmed-meshheading:8702849-Humans,
pubmed-meshheading:8702849-Interleukin-2,
pubmed-meshheading:8702849-Molecular Sequence Data,
pubmed-meshheading:8702849-Multigene Family,
pubmed-meshheading:8702849-NFATC Transcription Factors,
pubmed-meshheading:8702849-Nuclear Proteins,
pubmed-meshheading:8702849-Promoter Regions, Genetic,
pubmed-meshheading:8702849-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:8702849-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:8702849-RNA, Messenger,
pubmed-meshheading:8702849-Sequence Deletion,
pubmed-meshheading:8702849-Structure-Activity Relationship,
pubmed-meshheading:8702849-Transcription Factor AP-1,
pubmed-meshheading:8702849-Transcription Factors,
pubmed-meshheading:8702849-Tumor Cells, Cultured,
pubmed-meshheading:8702849-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
Characterization of a new isoform of the NFAT (nuclear factor of activated T cells) gene family member NFATc.
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pubmed:affiliation |
Department of Pathology, Roger Williams Medical Center-Brown University, Providence, Rhode Island 02908, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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