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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
31
|
| pubmed:dateCreated |
1996-9-24
|
| pubmed:databankReference | |
| pubmed:abstractText |
Fibronectin is an extracellular matrix glycoprotein encoded by a single gene. Alternative RNA splicing has been reported at three sites, ED (extra type III domain)-A, ED-B, and the variable or V region. Articular cartilage fibronectin monomers are rarely (ED-A)+, but approximately 25% are (ED-B)+. RNA gel electrophoresis and Northern blot analysis identified two (ED-B)+ and two (ED-B)- fibronectin transcripts in cartilage, each pair differing by approximately 750 bases. This difference results from a previously unreported RNA splicing pattern that eliminates not only the V region but also nucleotides encoding protein segments III-15 and I-10. This new splice variant, which we designate (V+C)-, represents the majority of fibronectin transcripts in equine, canine, and rabbit articular cartilage but is absent in the liver. Reverse transcriptase-polymerase chain reaction analyses of 11 additional equine tissues failed to detect the (V+C)- splice variant, except for very low levels in lymph node, bone, aorta, and skin. Furthermore, chondrocytes grown in monolayer culture maintain high levels of fibronectin expression but stop expressing (V+C)- transcripts over time. The tissue-specific expression pattern of this novel fibronectin isoform suggests that it may have an important function in the matrix organization of cartilage.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
2
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
18954-60
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8702559-Alternative Splicing,
pubmed-meshheading:8702559-Amino Acid Sequence,
pubmed-meshheading:8702559-Animals,
pubmed-meshheading:8702559-Base Sequence,
pubmed-meshheading:8702559-Cartilage, Articular,
pubmed-meshheading:8702559-DNA, Complementary,
pubmed-meshheading:8702559-DNA Primers,
pubmed-meshheading:8702559-Dogs,
pubmed-meshheading:8702559-Fibronectins,
pubmed-meshheading:8702559-Genetic Variation,
pubmed-meshheading:8702559-Horses,
pubmed-meshheading:8702559-Molecular Sequence Data,
pubmed-meshheading:8702559-Molecular Structure,
pubmed-meshheading:8702559-Polymerase Chain Reaction,
pubmed-meshheading:8702559-RNA, Messenger,
pubmed-meshheading:8702559-Rabbits,
pubmed-meshheading:8702559-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8702559-Species Specificity
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Fibronectin mRNA splice variant in articular cartilage lacks bases encoding the V, III-15, and I-10 protein segments.
|
| pubmed:affiliation |
Department of Physiology, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|