Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-8-30
pubmed:databankReference
pubmed:abstractText
A cDNA encoding a novel organic cation transporter, designated as OCT2 was isolated from the rat kidney. The rat OCT2 cDNA (2,205 bp) had an open reading frame encoding for a 593-amino acid protein (calculated molecular mass of 66 kDa) which shows 67% identity with the rat OCT1. Northern hybridization and reverse transcription-PCR analyses revealed that the rat OCT2 mRNA transcript was expressed predominantly in the kidney, at higher level in the medulla than in the cortex, but this transcript was not detected in the brain, heart, lung, liver, small intestine or spleen. When expressed in Xenopus oocytes, rat OCT2 stimulated the uptake of tetraethylammonium, and the uptake was markedly inhibited by the presence of cimetidine, procainamide and quinidine. These findings suggest that OCT2 is responsible for the transport of cationic drugs in the kidney.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cimetidine, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Organic Cation Transporter 1, http://linkedlifedata.com/resource/pubmed/chemical/Procainamide, http://linkedlifedata.com/resource/pubmed/chemical/Quinidine, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium, http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium Compounds
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
224
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
500-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8702418-Amino Acid Sequence, pubmed-meshheading:8702418-Animals, pubmed-meshheading:8702418-Base Sequence, pubmed-meshheading:8702418-Biological Transport, pubmed-meshheading:8702418-Blotting, Northern, pubmed-meshheading:8702418-Carrier Proteins, pubmed-meshheading:8702418-Cimetidine, pubmed-meshheading:8702418-Cloning, Molecular, pubmed-meshheading:8702418-DNA, Complementary, pubmed-meshheading:8702418-DNA Primers, pubmed-meshheading:8702418-Female, pubmed-meshheading:8702418-Kidney, pubmed-meshheading:8702418-Membrane Proteins, pubmed-meshheading:8702418-Molecular Sequence Data, pubmed-meshheading:8702418-Oocytes, pubmed-meshheading:8702418-Organic Cation Transport Proteins, pubmed-meshheading:8702418-Organic Cation Transporter 1, pubmed-meshheading:8702418-Polymerase Chain Reaction, pubmed-meshheading:8702418-Procainamide, pubmed-meshheading:8702418-Quinidine, pubmed-meshheading:8702418-RNA, Messenger, pubmed-meshheading:8702418-Rats, pubmed-meshheading:8702418-Recombinant Proteins, pubmed-meshheading:8702418-Sequence Homology, Amino Acid, pubmed-meshheading:8702418-Tetraethylammonium, pubmed-meshheading:8702418-Tetraethylammonium Compounds, pubmed-meshheading:8702418-Xenopus laevis
pubmed:year
1996
pubmed:articleTitle
cDNA cloning and functional expression of a novel rat kidney organic cation transporter, OCT2.
pubmed:affiliation
Department of Pharmacy, Kyoto University Hospital, Faculty of Medicine, Kyoto University, Japan.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't