Linked Life Data

8702268

Source:http://linkedlifedata.com/resource/pubmed/id/8702268

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-9-5
pubmed:databankReference
pubmed:abstractText
The enzymes phosphotransacetylase (PTA) and acetate kinase (AK) catalyze the conversion of acetyl coenzyme A to acetate in the fermentation of Clostridium acetobutylicum. The acetate-producing step is an important element in the acidogenic fermentation stage and generates ATP for clostridial cell growth. The genes pta and ack, encoding PTA and AK, respectively, were cloned and sequenced. Enzyme activity assays were performed on cell extracts from Escherichia coli and C. acetobutylicum harboring the subclone, and both AK and PTA activities were shown to be elevated. DNA sequence analysis showed that the pta and ack genes are adjacent in the clostridial chromosome, with pta upstream. The pta gene encodes a protein of 333 amino acid residues with a calculated molecular mass of 36.2 kDa, and ack encodes a polypeptide of 401 residues with a molecular mass of 44.3 kDa. Primer extension analysis identified a single transcriptional start site located 70 bp upstream of the start codon for the pta gene, suggesting an operon arrangement for these tandem genes. The results from overexpression of ack and pta in C. acetobutylicum showed that the final ratios of acetate to other major products were higher and that there was a greater proportion of two- versus four-carbon-derived products.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-13437487, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-1366944, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-1368230, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-1385386, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-16346566, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-16559158, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-21941, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2199603, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2383002, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2536666, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2719475, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2808380, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2844725, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2844814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-3020034, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-3027059, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-322279, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-3287331, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-3540574, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-4370922, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-4447427, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-6252971, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-6268170, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-6329717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7287757, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7542800, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7569993, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7883769, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7896690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7918659, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-7934828, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8010697, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8010698, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8226623, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8226682, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8244020, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8386500, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8396545, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-8669898, http://linkedlifedata.com/resource/pubmed/commentcorrection/8702268-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2758-66
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824.
pubmed:affiliation
Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.