Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-9-4
pubmed:abstractText
Ovarian cancers from 64 midwestern US women were screened for p53 dysfunction both by immunohistochemical staining (IHCS) and single strand conformation polymorphism (SSCP) analysis of the entire open reading frame (ORF). Forty SSCP abnormalities in 39 tumors included nine deletion, one insertion, two splice junction, two nonsense, one silent and 25 missense mutations were confirmed by direct genomic sequencing. Eight of the insertion/deletion defects may have occurred due to slippage during the course of DNA replication. This observation suggests that genomic instability may play an important role in ovarian carcinogenesis. Fifteen percent of the mutations encountered were located outside exons 5-9 and four of these were null. The sensitivity of IHCS was 96% for missense mutations but only 14% for null mutations. This contrasted with 100% sensitivity of the SSCP screening methodology. The 21% overall incidence of null mutations in the present study far exceeds the reported 6.8% incidence in the world literature (P=0.0003). Explanations for this difference include: (1) our complete analysis of the entire ORF of the p53 gene; (2) the tendency of others to rely upon IHCS to screen tumors prior to mutation analysis; and (3) environmental or endogenous genetic influences.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
4
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-23
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8700537-Adult, pubmed-meshheading:8700537-Aged, pubmed-meshheading:8700537-Aged, 80 and over, pubmed-meshheading:8700537-Base Sequence, pubmed-meshheading:8700537-DNA, Neoplasm, pubmed-meshheading:8700537-DNA Mutational Analysis, pubmed-meshheading:8700537-DNA Replication, pubmed-meshheading:8700537-Female, pubmed-meshheading:8700537-Genes, p53, pubmed-meshheading:8700537-Humans, pubmed-meshheading:8700537-Immunoenzyme Techniques, pubmed-meshheading:8700537-Middle Aged, pubmed-meshheading:8700537-Molecular Sequence Data, pubmed-meshheading:8700537-Mutation, pubmed-meshheading:8700537-Neoplasm Proteins, pubmed-meshheading:8700537-Open Reading Frames, pubmed-meshheading:8700537-Ovarian Neoplasms, pubmed-meshheading:8700537-Point Mutation, pubmed-meshheading:8700537-Polymerase Chain Reaction, pubmed-meshheading:8700537-Polymorphism, Single-Stranded Conformational, pubmed-meshheading:8700537-Sensitivity and Specificity, pubmed-meshheading:8700537-Sequence Deletion, pubmed-meshheading:8700537-Tumor Suppressor Protein p53
pubmed:year
1996
pubmed:articleTitle
An abundance of p53 null mutations in ovarian carcinoma.
pubmed:affiliation
Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, 52242, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't