Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-9-5
|
| pubmed:abstractText |
A histochemical study using lectins to identify glycoconjugates present in the efferent ducts and ductus epididymidis of men without testicular or related disease was carried out. The lectins used and the oligosaccharide residues linked were: wheat germ agglutinin (WGA) for beta-N-acetylglucosamine and sialic acid, concanavalin A (ConA) for alpha-mannose, Ulex europaeus agglutinin (UEA-I) for alpha-fucose, Dolichos biflorus agglutinin (DBA) for alpha-N-acetylgalactosamine, soy bean agglutinin (SBA) for beta-N-acetylgalactosamine, and peanut agglutinin (PNA) for beta-galactose. The lectin-binding pattern in the human epididymis presents similarities and differences to those observed in other mammals which also showed differences between species. The present results revealed that regional differences along the human ductus epididymidis were less pronounced than those reported in other mammals. The epithelial cells in the efferent ducts reacted positively to WGA. All along the length of the ductus epididymidis, the principal cells and the luminal content showed staining affinity for WGA and ConA. The epididymal principal cells and luminal content also reacted positively to DBA for alpha-N-acetylgalactosamine but only in the cauda epididymidis. A positive reaction to UEA-I was observed only in the luminal content of the cauda epididymidis. This finding suggests that changes in the oligosaccharide chains of secretions leading to a positive UEA-I reaction occur in the cauda epididymidis. The epididymal principal cells showed positive reactions to SBA and PNA over the apical microvilli but not in the cytoplasm. The reaction was observed in the caput and corpus epididymidis but not in the cauda. Positive reactions to SBA and PNA were also detected in the epididymal fluid and in the cytoplasm of mitochondria-rich cells (a minor population of epididymal epithelial cells). These cells also reacted to other lectins such as WGA, ConA and DBA, which were also detected in the principal cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylgalactosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Acetylglucosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/DB58 lectin, Dolichos biflorus,
http://linkedlifedata.com/resource/pubmed/chemical/Fucose,
http://linkedlifedata.com/resource/pubmed/chemical/Galactose,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Mannose,
http://linkedlifedata.com/resource/pubmed/chemical/N-Acetylneuraminic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Peanut Agglutinin,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Soybean Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ulex europaeus lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Wheat Germ Agglutinins,
http://linkedlifedata.com/resource/pubmed/chemical/soybean lectin
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-4251
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
106
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
313-20
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8699416-Acetylgalactosamine,
pubmed-meshheading:8699416-Acetylglucosamine,
pubmed-meshheading:8699416-Adult,
pubmed-meshheading:8699416-Arachis hypogaea,
pubmed-meshheading:8699416-Concanavalin A,
pubmed-meshheading:8699416-Epididymis,
pubmed-meshheading:8699416-Fucose,
pubmed-meshheading:8699416-Galactose,
pubmed-meshheading:8699416-Glycoconjugates,
pubmed-meshheading:8699416-Histocytochemistry,
pubmed-meshheading:8699416-Humans,
pubmed-meshheading:8699416-Lectins,
pubmed-meshheading:8699416-Male,
pubmed-meshheading:8699416-Mannose,
pubmed-meshheading:8699416-Middle Aged,
pubmed-meshheading:8699416-N-Acetylneuraminic Acid,
pubmed-meshheading:8699416-Peanut Agglutinin,
pubmed-meshheading:8699416-Plant Lectins,
pubmed-meshheading:8699416-Sialic Acids,
pubmed-meshheading:8699416-Soybean Proteins,
pubmed-meshheading:8699416-Soybeans,
pubmed-meshheading:8699416-Wheat Germ Agglutinins
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Lectin histochemistry in the human epididymis.
|
| pubmed:affiliation |
Department of Cell Biology and Genetics, University of Alcalá de Henares, Madrid, Spain.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|