Linked Life Data

8697636

Source:http://linkedlifedata.com/resource/pubmed/id/8697636

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-9-3
pubmed:abstractText
In this study we have evaluated the prevalence of antibodies against core region peptides (residues 1-28, 21-38 and 51-68), the envelope 1, the non-structural (NS) 4 and 5 proteins of hepatitis C virus (HCV) in sera from 65 chronically HCV-infected patients, 47 with mixed cryoglobulinaemia (MC+) and 18 without (MC-). The major binding sites were located within the core region. Regions 1-28 and 51-68 were recognized by a similar proportion of MC+ and MC- patients, while peptide 21-38 was less frequently detected by samples from MC+ patients (65.5% versus 100%, P = 0.011). The patterns of the reactions showed a minimum of three binding sites: one, located within region 51-68, was shared by both groups; a second determinant was identified at residues 1-21 for MC+ patients and at residues 28-38 for MC- patients; a third, not exactly localized, lay between residues 1 and 38. Recognition of NS5 peptides was not significantly different between MC+ and MC- patients, but while the former mostly reacted either with peptide 1 (residues 2294-2309) (five of 15 sera) or with peptide 2 (residues 2304-2319) (nine of 15 sera), the latter group showed a more scattered reaction. Antibodies to HCV peptides prevalently belonged to IgGl subclass. However, whereas IgGl antibodies against peptide 21-38 and peptide 1 of NS5 were more frequently found in MC- rather than in MC+ patients (100% versus 63.8%, P = 0.003, and 22.2% versus 4.2%, P = 0.025, respectively), IgG3 antibodies against region 1-28 were more frequent in MC+ patients (53.19% versus 16.6%, P = 0.0078). Overall, the data suggest that a differential humoral immune response to HCV antigens occurs in patients with and without cryoglobulinaemia.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0009-9104
pubmed:author
pubmed:issnType
Print
pubmed:volume
105
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
59-64
pubmed:dateRevised
2008-11-20
pubmed:meshHeading
pubmed-meshheading:8697636-Adult, pubmed-meshheading:8697636-Aged, pubmed-meshheading:8697636-Base Sequence, pubmed-meshheading:8697636-Cryoglobulinemia, pubmed-meshheading:8697636-Female, pubmed-meshheading:8697636-Genotype, pubmed-meshheading:8697636-Hepacivirus, pubmed-meshheading:8697636-Hepatitis C, pubmed-meshheading:8697636-Hepatitis C Antibodies, pubmed-meshheading:8697636-Hepatitis C Antigens, pubmed-meshheading:8697636-Humans, pubmed-meshheading:8697636-Immunoglobulin G, pubmed-meshheading:8697636-Male, pubmed-meshheading:8697636-Middle Aged, pubmed-meshheading:8697636-Molecular Sequence Data, pubmed-meshheading:8697636-Peptides, pubmed-meshheading:8697636-Viral Core Proteins, pubmed-meshheading:8697636-Viral Envelope Proteins, pubmed-meshheading:8697636-Viral Nonstructural Proteins
pubmed:year
1996
pubmed:articleTitle
Differential humoral immune response against hepatitis C virus antigenic synthetic peptides in infected patients with and without mixed cryoglobulinaemia.
pubmed:affiliation
Istituto di Clinica Medica Generale, Ematologia ed Immunologia Clinica, Università di Ancona, Italy.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't