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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-8-29
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pubmed:abstractText |
Interleukin-12 is a heterodimeric cytokine, mainly produced by macrophages. In our present study we demonstrate that interleukin-12 expression is regulated by nitric oxide. Incubation of the macrophage cell line IC 21 with interferon-gamma gave rise to both interleukin-12 p40 mRNA and nitric oxide production. The concurrent addition of the nitric oxide synthase inhibitor N(G)-monomethyl-L-arginine inhibited nitrite production and in parallel completely suppressed interleukin-12 p40 mRNA formation. This indicated that endogenous nitric oxide synthase activity was required for IL-12 p40 gene expression. Exposure of the cells towards the nitric oxide generating compounds nitroprusside or S-nitroso-N-acetyl-penicillamine induced interleukin-12 p40 mRNA. Maximal mRNA levels were induced with nitric oxide donors at 1 microM concentration. We conclude that nitric oxide may exert an autoregulatory and paracrine control of interleukin-12 gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/S-Nitroso-N-Acetylpenicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/omega-N-Methylarginine
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
224
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-63
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8694804-Animals,
pubmed-meshheading:8694804-Arginine,
pubmed-meshheading:8694804-Cell Line,
pubmed-meshheading:8694804-Enzyme Inhibitors,
pubmed-meshheading:8694804-Gene Expression Regulation,
pubmed-meshheading:8694804-Interferon-gamma,
pubmed-meshheading:8694804-Interleukin-12,
pubmed-meshheading:8694804-Macrophage Activation,
pubmed-meshheading:8694804-Macrophages,
pubmed-meshheading:8694804-Mice,
pubmed-meshheading:8694804-Nitric Oxide,
pubmed-meshheading:8694804-Nitric Oxide Synthase,
pubmed-meshheading:8694804-Nitroprusside,
pubmed-meshheading:8694804-Penicillamine,
pubmed-meshheading:8694804-RNA, Messenger,
pubmed-meshheading:8694804-Rats,
pubmed-meshheading:8694804-Recombinant Proteins,
pubmed-meshheading:8694804-S-Nitroso-N-Acetylpenicillamine,
pubmed-meshheading:8694804-Transcription, Genetic,
pubmed-meshheading:8694804-omega-N-Methylarginine
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pubmed:year |
1996
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pubmed:articleTitle |
Interleukin-12 gene-expression of macrophages is regulated by nitric oxide.
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pubmed:affiliation |
Diabetes Research Institute, Heinrich- Heine University of Düsseldorf, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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