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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-8-23
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pubmed:abstractText |
Two enzymes in the methionine salvage pathway, 5-methylthioribose kinase (MTR kinase) and 5'-methylthioadenosine/ S-adenosylhomocysteine nucleosidase (MTA/SAH nucleosidase) were purified from Klebsiella pneumoniae. Chromatography using a novel 5'-(p-aminophenyl)thioadenosine/5-(p-aminophenyl)thioribose affinity matrix allowed the binding and selective elution of each of the enzymes in pure form. The molecular mass, substrate kinetics and N-terminal amino acid sequences were characterized for each of the enzymes. Purified MTR kinase exhibits an apparent molecular mass of 46-50 kDa by SDS/PAGE and S200HR chromatography, and has a Km for MTR of 12.2 microM. Homogeneous MTA/SAH nucleosidase displays a molecular mass of 26.5 kDa by SDS/PAGE, and a Km for MTA of 8.7 microM. Comparisons of the N-terminal sequences obtained for each of the enzymes with protein-sequence databases failed to reveal any significant sequence similarities to known proteins. However, the amino acid sequence obtained for the nucleosidase did share a high degree of sequence similarity with the putative translation product of an open reading frame in Escherichia coli, thus providing a tentative identification of this gene as encoding an MTA/SAH nucleosidase.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-1245911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-15463143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-16662931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-16664444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-1929327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-210167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-2153115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-2157212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-2211600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-2854458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-2855560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-3911944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-4203512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-428559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-4562670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6293478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6312270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6351723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6405586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6783408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6803789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-6991690,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-7920254,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-8393067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-850235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8694776-901760
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
317 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-90
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
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pubmed:year |
1996
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pubmed:articleTitle |
Affinity purification of 5-methylthioribose kinase and 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase from Klebsiella pneumoniae [corrected].
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Oregon Health Sciences University, Portland 97201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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