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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
14
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pubmed:dateCreated |
1996-8-29
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pubmed:abstractText |
The binding stoichiometry of gene V protein from bacteriophage f1 to several oligonucleotides was studied using electrospray ionization-mass spectrometry (ESI-MS). Using mild mass spectrometer interface conditions that preserve noncovalent associations in solution, gene V protein was observed as dimer ions from a 10 mM NH4OAc solution. Addition of oligonucleotides resulted in formation of protein-oligonucleotide complexes with stoichiometry of approximately four nucleotides (nt) per protein monomer. A 16-mer oligonucleotide gave predominantly a 4:1 (protein monomer: oligonucleotide) complex while oligonucleotides shorter than 15 nt showed stoichiometries of 2:1. Stoichiometries and relative binding constants for a mixture of oligonucleotides were readily measured using mass spectrometry. The binding stoichiometry of the protein with the 16-mer oligonucleotide was measured independently using size-exclusion chromatography and the results were consistent with the mass spectrometric data. These results demonstrate, for the first time, the observation and stoichiometric measurement of protein-oligonucleotide complexes using ESI-MS. The sensitivity and high resolution of ESI-MS should make it a useful too] in the study of protein-DNA interactions.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-1729248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-1761038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-1928723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2007116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2194402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2482044,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2657734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2671388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-2675315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-3054122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-3271425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-3890949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-4115107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-4747632,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-4939035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-4943754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6243083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6365163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6601193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6684697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6749299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-6981459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-7046792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-781261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-8134350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8692937-8461341
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
9
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7022-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8692937-Base Sequence,
pubmed-meshheading:8692937-DNA-Binding Proteins,
pubmed-meshheading:8692937-Macromolecular Substances,
pubmed-meshheading:8692937-Mass Spectrometry,
pubmed-meshheading:8692937-Molecular Sequence Data,
pubmed-meshheading:8692937-Oligodeoxyribonucleotides,
pubmed-meshheading:8692937-Poly A,
pubmed-meshheading:8692937-Poly T,
pubmed-meshheading:8692937-Protein Binding,
pubmed-meshheading:8692937-Structure-Activity Relationship,
pubmed-meshheading:8692937-Viral Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Direct measurement of oligonucleotide binding stoichiometry of gene V protein by mass spectrometry.
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pubmed:affiliation |
Environmental Molecular Sciences laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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