Linked Life Data

8690723

Source:http://linkedlifedata.com/resource/pubmed/id/8690723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-8-26
pubmed:abstractText
Cis-acting elements of the gene for mouse glycophorin, an erythroid-specific membrane glycoprotein, were determined by transient and stable transfection assays using murine erythroleukemia (MEL) cells. Cis-acting elements proximal to the transcription start site of the gene can be separated into the basal promoter (-1 to 191 bp) and the distal element (-133 to -92). The basal promoter contained GGTGG and GATA motifs and the distal element contained GATA-1 and NF-E2 motifs. Deletion analysis of the distal GATA site and its neighboring sequence and DNase-I footprinting/EMSA (electrophoretic mobility shift assay) analysis indicated that induced nuclear factor binding to GATA-1 and its neighboring sequence may be required for expression during MEL cell differentiation induced by dimethyl sulfoxide treatment. The NF-E2 site was also shown to be essential for the promoter activity. An approximately 400 bp far upstream region (-1325 to -948bp) containing the binding motifs for GGGTGG, GATA-1 and NF-E2 showed no enhancing activity when this region was examined by transient transfection assay, but it did show enhancement of the differentiation-specific promoter activity in the stable transfection assay. The far upstream region of mouse glycophorin gene may have a function similar to that of the locus control region (LCR) of human beta-globulin gene cluster.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
118
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
593-600
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed-meshheading:8690723-Animals, pubmed-meshheading:8690723-Base Sequence, pubmed-meshheading:8690723-Binding Sites, pubmed-meshheading:8690723-Cell Differentiation, pubmed-meshheading:8690723-DNA-Binding Proteins, pubmed-meshheading:8690723-Gene Expression Regulation, Leukemic, pubmed-meshheading:8690723-Globins, pubmed-meshheading:8690723-Glycophorin, pubmed-meshheading:8690723-Humans, pubmed-meshheading:8690723-Internal-External Control, pubmed-meshheading:8690723-Leukemia, Erythroblastic, Acute, pubmed-meshheading:8690723-Mice, pubmed-meshheading:8690723-Molecular Sequence Data, pubmed-meshheading:8690723-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8690723-Sequence Homology, Nucleic Acid, pubmed-meshheading:8690723-Transfection, pubmed-meshheading:8690723-Tumor Cells, Cultured
pubmed:year
1995
pubmed:articleTitle
Inducible expression of erythroid-specific mouse glycophorin gene is regulated by proximal elements and locus control region-like sequence.
pubmed:affiliation
Department of Cell Biology, Tohoku University, Sendai.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't