Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-8-29
|
| pubmed:abstractText |
Malignant lymphomas frequently develop in the pleural cavity of patients with long-standing pyothorax. The term pyothorax-associated lymphoma (PAL) has been proposed for this type of tumor. Most PALs are diffuse lymphomas of the B-cell type and contain Epstein-Barr virus (EBV) DNA. We have established 2 lymphoma cell lines from biopsy specimens of PAL cases, OPL-1 and OPL-2, and examined their growth characteristics and the expression of EBV latent infection genes and oncogenes. OPL-2 exhibited a more rapid growth and higher saturation density than OPL-1, and only OPL-2 exhibited colony-forming activity in soft agar. OPL-1 and -2 were positive for B-cell differentiation markers and showed clonal surface immunoglobulins. Both line contained a single predominant form of episomal EBV DNA, indicating clonal cellular proliferation of an EBV-infected progenitor cell. OPL-1 and -2 contained type B and A EBV genome, respectively. Expression of EBV nuclear antigen (EBNA)2 mRNA and protein was detected by Northern and Western blot analysis in OPL-1, but not in OPL-2. On the other hand, the expression of latent membrane protein (LMP)1 mRNA in both OPL-1 and -2 was extremely weak and detectable only by reverse transcription-polymerase chain reaction. Protein expression of LMP1 was not observed by Western blot analysis or immunocytochemistry. Both lines expressed c-myc mRNA. Only OPL-1 expressed mRNA of c-fgr, an oncogene whose expression is upregulated by EBNA2. Both OPLs expressed bcl-2 mRNA without detectable expression of LMP1 protein.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/EBV-associated membrane antigen...,
http://linkedlifedata.com/resource/pubmed/chemical/Epstein-Barr Virus Nuclear Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Matrix Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0020-7136
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
3
|
| pubmed:volume |
67
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
86-94
|
| pubmed:dateRevised |
2007-7-24
|
| pubmed:meshHeading |
pubmed-meshheading:8690530-Aged,
pubmed-meshheading:8690530-Antigens, Viral,
pubmed-meshheading:8690530-Base Sequence,
pubmed-meshheading:8690530-Chromosome Aberrations,
pubmed-meshheading:8690530-DNA-Binding Proteins,
pubmed-meshheading:8690530-Empyema, Pleural,
pubmed-meshheading:8690530-Epstein-Barr Virus Nuclear Antigens,
pubmed-meshheading:8690530-Gene Rearrangement,
pubmed-meshheading:8690530-Genes, Immunoglobulin,
pubmed-meshheading:8690530-Genome, Viral,
pubmed-meshheading:8690530-Herpesvirus 4, Human,
pubmed-meshheading:8690530-Humans,
pubmed-meshheading:8690530-Immunophenotyping,
pubmed-meshheading:8690530-Lymphoma,
pubmed-meshheading:8690530-Male,
pubmed-meshheading:8690530-Molecular Sequence Data,
pubmed-meshheading:8690530-Oncogenes,
pubmed-meshheading:8690530-Tumor Cells, Cultured,
pubmed-meshheading:8690530-Viral Matrix Proteins
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Expression of Epstein-Barr virus latent infection genes and oncogenes in lymphoma cell lines derived from pyothorax-associated lymphoma.
|
| pubmed:affiliation |
Departmemt of Pathology, Osaka University Medical School, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|