|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
8
|
|
pubmed:dateCreated |
1996-8-23
|
|
pubmed:abstractText |
Interleukin-11 (IL-11), which has been detected as a stromal cell-derived cytokine, regulates multiple steps of early hematopoiesis. Synthesis of IL-11 is induced by IL-1 and transforming growth factor-beta (TGF-beta) in stromal cells and fibroblasts, but the cytokines that inhibit its production remain to be defined. In the present study, we demonstrate that interferon-alpha (INF-alpha) downregulates IL-1-induced IL-11 in human bone marrow stromal cultures. In Northern blot experiments, expression of IL-11 mRNA was reduced in the presence of INF-alpha; this inhibiton was prevented by the addition of cycloheximide. In eight independent experiments, IL-1-stimulated production of IL-11 was significantly inhibited by INF-alpha (p = 0.0117) as measured in stromal cell supernatants by specific enzyme-linked immunosorbent assay (ELISA). Dose titration experiments revealed a dose-dependent inhibition already beginning at a dose level of 10 U/mL IFN. These results are in keeping with our previous observation defining an inhibitory role of INF-alpha in the production of hematopoietic growth factors produced by the bone marrow microenvironment.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jul
|
|
pubmed:issn |
0301-472X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
863-7
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:8690043-Bone Marrow,
pubmed-meshheading:8690043-Bone Marrow Cells,
pubmed-meshheading:8690043-Cells, Cultured,
pubmed-meshheading:8690043-Cycloheximide,
pubmed-meshheading:8690043-Dose-Response Relationship, Drug,
pubmed-meshheading:8690043-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8690043-Humans,
pubmed-meshheading:8690043-Interferon-alpha,
pubmed-meshheading:8690043-Interleukin-1,
pubmed-meshheading:8690043-Interleukin-11,
pubmed-meshheading:8690043-Kinetics,
pubmed-meshheading:8690043-Lipopolysaccharides,
pubmed-meshheading:8690043-RNA, Messenger,
pubmed-meshheading:8690043-Recombinant Proteins,
pubmed-meshheading:8690043-Stromal Cells,
pubmed-meshheading:8690043-Transcription, Genetic,
pubmed-meshheading:8690043-Tumor Necrosis Factor-alpha
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Inhibition of interleukin-11 by interferon-alpha in human bone marrow stromal cells.
|
|
pubmed:affiliation |
III Department of Internal Medicine, Johannes-Gutenberg-University, School of Medicine, Mainz, Germany.
|
|
pubmed:publicationType |
Journal Article
|
