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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-8-20
|
| pubmed:abstractText |
A subset of gastric carcinoma carries Epstein-Barr virus (EBV). The immunophenotypic features of EBV-associated (EBV+) gastric carcinoma, which we have analyzed using 25 EBV+ cases, remain unclear. Frozen tissue samples were stained with antibodies to various immune cell markers. To evaluate the proliferative activity of CD8+ cells, we performed CD8/Ki-67 double staining on paraffin-embedded sections. The results were compared with those in EBV-negative (EBV-) gastric carcinomas. All EBV+ and EBV- gastric carcinoma cells expressed major histocompatibility complex class I, whereas major histocompatibility complex class II expression in tumor cells was more prominent in EBV+ cases. Intercellular adhesion molecule-1 and Fas/APO-1 expression was largely restricted to EBV+ cases. The lymphocytes that infiltrated EBV+ tumor nests were predominantly CD8+ T cells, many of which expressed perforin. Immunoelectron microscopy confirmed a close cell to cell contact between these CD8+ cells and carcinoma cells. CD8+ cells were CD11a+ and CD11b- by flow cytometry performed in one case. The labeling index of Ki-67, the proliferation-associated antigen, in CD8+ cells was 4 times higher in EBV+ cases than in EBV- cases. Our data suggest that these CD8+ cells, which bear a cytotoxic phenotype, are actively proliferating in close contact with EBV+ tumor cells and that the specificity of the CD8+ cells may be directed to EBV and/or cellular antigens expressed by the tumor. This is consistent with a generally favorable prognosis of EBV+ gastric carcinoma. Because the observed T-cell infiltration is insufficient to eradicate the tumor cells, certain immunosuppressive factors were speculated to allow the essentially immunogenic carcinoma cells to establish a macroscopic lesion.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0023-6837
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
75
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
67-76
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8683941-Antigens, CD,
pubmed-meshheading:8683941-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8683941-Cell Adhesion Molecules,
pubmed-meshheading:8683941-Cell Count,
pubmed-meshheading:8683941-Cell Division,
pubmed-meshheading:8683941-Flow Cytometry,
pubmed-meshheading:8683941-Herpesviridae Infections,
pubmed-meshheading:8683941-Herpesvirus 4, Human,
pubmed-meshheading:8683941-Humans,
pubmed-meshheading:8683941-Immunohistochemistry,
pubmed-meshheading:8683941-Immunophenotyping,
pubmed-meshheading:8683941-In Situ Hybridization,
pubmed-meshheading:8683941-Microscopy, Immunoelectron,
pubmed-meshheading:8683941-Neoplasm Invasiveness,
pubmed-meshheading:8683941-Stomach Neoplasms,
pubmed-meshheading:8683941-Tumor Markers, Biological,
pubmed-meshheading:8683941-Tumor Virus Infections
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Immunophenotypic characterization of Epstein-Barr virus-associated gastric carcinoma: massive infiltration by proliferating CD8+ T-lymphocytes.
|
| pubmed:affiliation |
Department of Pathology, Tohoku University School of Medicine, Aoba-ku, Sendai, Japan.
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| pubmed:publicationType |
Journal Article
|