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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1996-8-19
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pubmed:abstractText |
1. A small-conductance Cl- channel that is stimulated by ATP and protein kinase A has been identified in the basolateral membranes of cortical thick ascending limbs (CTALs) of the mouse nephron. The present study uses the cell-attached and inside-out variants of the patch-clamp technique to investigate the pH sensitivity of this channel. 2. The open-state probability (Po) was dependent upon the internal pH in inside-out patches. Expressed as a percentage of the Po value at pH 7.2, Po increased to about 180% at pH 7.6, and decreased to 25% at pH 6.8. Po was close to zero at pH 6.4. The internal pH had no effect on the channel unit conductance. 3. The effect of pH on the CTAL Cl- channel was assessed in intact cells using NH4Cl to acidify the intracellular compartment. Experiments with the pH-sensitive fluorescent dye 2',7'-(carboxyethyl)-5'(6')-carboxy fluorescein penta-acetoxymethyl ester (BCECF) indicated that 1 mmol l-1 NH4Cl acidified the cytoplasm by 0.15 pH units and 5 mmol l-1 NH4Cl by 0.34 pH units. These concentrations of NH4Cl reduced the activity of the CTAL Cl- channel by 24 and 82% in cell-attached patches, showing that moderate changes in internal pH substantially altered the activity of this channel. NH4+ had no direct effect on channel activity. 4. Inhibition at low pH is a newly discovered property of small-conductance Cl- channels in epithelia, which might help discriminate between types of Cl- channel.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1375035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1378835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1384347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1588302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1692341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1712081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-1718002,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-2315005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-2643123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-36128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-3771799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7522329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7658386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7761259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7814604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7840147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-7938226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-8041726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-8113415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8683473-8184886
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-3751
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
490 ( Pt 3)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
759-65
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8683473-Animals,
pubmed-meshheading:8683473-Chloride Channels,
pubmed-meshheading:8683473-Cyclic AMP,
pubmed-meshheading:8683473-Hydrogen-Ion Concentration,
pubmed-meshheading:8683473-Kinetics,
pubmed-meshheading:8683473-Male,
pubmed-meshheading:8683473-Membrane Potentials,
pubmed-meshheading:8683473-Mice,
pubmed-meshheading:8683473-Mice, Inbred Strains,
pubmed-meshheading:8683473-Nephrons
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pubmed:year |
1996
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pubmed:articleTitle |
Inhibition of a small-conductance cAMP-dependent Cl- channel in the mouse thick ascending limb at low internal pH.
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pubmed:affiliation |
INSERM Unité 323, Faculté de Médecine Necker Enfants-Malades, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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