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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-8-19
|
| pubmed:abstractText |
Ninety-four patients with transitional cell carcinoma (TCC) of the renal pelvis and ureter, including dysplastic lesions, were studied for p53 and bcl-2 protein expression by immunohistochemistry. Twenty-one patients were also studied for p53 gene mutations by direct sequencing and for bcl-2 gene rearrangement by Southern blot analysis. Overexpressed p53 protein was detected in 26 cases (27.7 per cent). bcl-2 immunostaining was observed in 21 tumours (22.3 per cent), including four cases with labelling for p53. Furthermore, the dysplastic lesions surrounding 19 p53-positive tumours also stained for p53. bcl-2 expression was also detected frequently in dysplastic lesions adjacent to 14 bcl-2-positive TCCs. Positive reactions of dysplastic lesions were also found adjacent to 37 bcl-2-negative tumours. p53 point mutation was detected in 6 of 21 cases. Five of the six cases were positive for p53 protein. blc-2 positivity was detected in 3 of 21 tumours, without bcl-2 gene rearrangements in the major breakpoint region. Overexpressed p53 protein was frequently detected in both high-grade (P < 0.05) and invasive tumours (P < 0.05). In three cases of p53-positive non-papillary invasive tumours, bcl-2 was found in non-invasive portions, but was not present in invasive areas. These findings suggest that overexpression (mutation) of p53 and/or bcl-2 protein may be early events in tumourigenesis and that p53 alterations in particular are essential for the maintenance of a malignant phenotype in tumour development.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3417
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
178
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
133-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8683378-Adult,
pubmed-meshheading:8683378-Aged,
pubmed-meshheading:8683378-Aged, 80 and over,
pubmed-meshheading:8683378-Base Sequence,
pubmed-meshheading:8683378-Blotting, Southern,
pubmed-meshheading:8683378-Carcinoma, Transitional Cell,
pubmed-meshheading:8683378-Female,
pubmed-meshheading:8683378-Genes, p53,
pubmed-meshheading:8683378-Humans,
pubmed-meshheading:8683378-Immunoenzyme Techniques,
pubmed-meshheading:8683378-Kidney Neoplasms,
pubmed-meshheading:8683378-Male,
pubmed-meshheading:8683378-Middle Aged,
pubmed-meshheading:8683378-Molecular Sequence Data,
pubmed-meshheading:8683378-Neoplasm Proteins,
pubmed-meshheading:8683378-Precancerous Conditions,
pubmed-meshheading:8683378-Proto-Oncogene Proteins,
pubmed-meshheading:8683378-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8683378-Tumor Suppressor Protein p53,
pubmed-meshheading:8683378-Ureteral Neoplasms
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Detection of p53 and bcl-2 protein in carcinoma of the renal pelvis and ureter including dysplasia.
|
| pubmed:affiliation |
Department of Pathology II, Kochi Medical School, Nankoku, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|