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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1996-8-20
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated suppressor mutants that suppress temperature-sensitive colony formation and anucleate cell production of a mukB mutation. A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E. All of the rne (smbB) mutants code for truncated RNase E polypeptides lacking a carboxyl-terminal half. The amount of MukB protein was higher in these rne mutants than that in the rne+ strain. These rne mutants grew nearly normally in the mukB+ genetic background. The copy number of plasmid pBR322 in these rne mutants was lower than that in the rne+ isogenic strain. The results suggest that these rne mutations increase the half-lives of mukB mRNA and RNAI of pBR322, the antisense RNA regulating ColE1-type plasmid replication. We have demonstrated that the wild-type RNase E protein bound to polynucleotide phosphorylase (PNPase) but a truncated RNase E polypeptide lacking the C-terminal half did not. We conclude that the C-terminal half of RNase E is not essential for viability but plays an important role for binding with PNPase. RNase E and PNPase of the multiprotein complex presumably cooperate for effective processing and turnover of specific substrates, such as mRNAs and other RNAs in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-110942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-13278318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1370457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1427102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1447125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1447789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1464330,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-149110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1497312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1704067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1704367,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1708438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1712252,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1925018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1943710,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-1989883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-2013571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-2199322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-2202593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-2414455,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-2645057,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-3061803,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-342528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-365352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-369943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-3897195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-4208895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-6327467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-7510217,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-7687578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-7721693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-7891559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-7988894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8182758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8320240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8412702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8415644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8514741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8566713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8568879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8682798-8602138
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Bacterial Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MukB protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Polyribonucleotide...,
http://linkedlifedata.com/resource/pubmed/chemical/ribonuclease E
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
178
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3917-25
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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