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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1996-8-15
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pubmed:abstractText |
We used CD28-deficient mice to analyze the importance of CD28 costimulation for the response against Staphylococcal enterotoxin B (SEB) in vivo. CD28 was necessary for the strong expansion of V beta 8+ T cells, but not for deletion. The lack of expansion was not due to a failure of SEB to activate V beta 8+ T cells, as V beta 8+ T cells from both CD28-/- and CD28+/+ mice showed similar phenotypic changes within the first 24 h after SEB injection and cell cycle analysis showed that an equal percentage of V beta 8+ T cells started to proliferate. However, the phenotype and the state of proliferation of V beta 8+ T cells was different at later time points. Furthermore, in CD28-/- mice injection with SEB led to rapid induction of unresponsiveness in SEB responsive T cells, indicated by a drastic reduction of proliferation after secondary SEB stimulation in vitro. Unresponsiveness could also be demonstrated in vivo, as CD28-/- mice produced only marginal amounts of TNF alpha after rechallenge with SEB. In addition CD28-/- mice were protected against a lethal toxic shock induced by a second injection with SEB. Our results indicate that CD28 costimulation is crucial for the T cell-mediated toxicity of SEB and demonstrate that T cell stimulation in the absence of CD28 costimulation induces unresponsiveness in vivo.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1313075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1313950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1335362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1346270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1372018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1418619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1670963,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1730929,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1832875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1907921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-1950767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2037017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2113314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2212944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2303780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2343314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2465550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2522086,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2540528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-2785644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-3259256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7522010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7534617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7539104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7540940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7621080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7688139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7751626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7760014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7774618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7777536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-7790813,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-8104798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-8225363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-8228806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8676068-8343954
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2481-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8676068-Animals,
pubmed-meshheading:8676068-Antigens, CD28,
pubmed-meshheading:8676068-Cells, Cultured,
pubmed-meshheading:8676068-Crosses, Genetic,
pubmed-meshheading:8676068-Enterotoxins,
pubmed-meshheading:8676068-Flow Cytometry,
pubmed-meshheading:8676068-H-2 Antigens,
pubmed-meshheading:8676068-Immunity, Innate,
pubmed-meshheading:8676068-Immunophenotyping,
pubmed-meshheading:8676068-Interleukin-2,
pubmed-meshheading:8676068-Kinetics,
pubmed-meshheading:8676068-Lymph Nodes,
pubmed-meshheading:8676068-Lymphocyte Activation,
pubmed-meshheading:8676068-Mice,
pubmed-meshheading:8676068-Mice, Inbred BALB C,
pubmed-meshheading:8676068-Mice, Knockout,
pubmed-meshheading:8676068-Shock, Septic,
pubmed-meshheading:8676068-Staphylococcus aureus,
pubmed-meshheading:8676068-Superantigens,
pubmed-meshheading:8676068-T-Lymphocytes,
pubmed-meshheading:8676068-Time Factors,
pubmed-meshheading:8676068-Tumor Necrosis Factor-alpha
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pubmed:year |
1996
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pubmed:articleTitle |
Induction of unresponsiveness and impaired T cell expansion by staphylococcal enterotoxin B in CD28-deficient mice.
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pubmed:affiliation |
Department of Immunology and Biophysics, Ontario Cancer Institute, University of Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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