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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1996-8-15
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pubmed:databankReference |
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pubmed:abstractText |
The 89-kDa STAT3 protein is a latent transcription factor which is activated in response to cytokines (interleukin (IL)-5 and -6) and growth factors (epidermal growth factor). Binding of IL-5 to its specific receptor activates JAK2 which leads to the tyrosine phosphorylation of STAT3 proteins. Here we report the cloning of a cDNA encoding a variant of the transcription factor STAT3 (named STAT3beta) which was isolated by screening an eosinophil cDNA library. Compared to wild-type STAT3, STAT3beta lacks an internal domain of 50 base pairs located near the C terminus. This splice product is a naturally occurring isoform of STAT3 and encodes a 80-kDa protein. We found by reconstitution of the human IL-5R in COS cells that like STAT3, STAT3beta is phosphorylated on tyrosine and binds to the pIRE from the ICAM-1 promoter after IL-5 stimulation. However, STAT3beta fails to activate a pIRE containing promoter in transient transfection assays. Instead, co-expression of STAT3beta inhibits the transactivation potential of STAT3. These results suggests that STAT3beta functions as a negative regulator of transcription.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13221-7
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:8675499-Amino Acid Sequence,
pubmed-meshheading:8675499-Animals,
pubmed-meshheading:8675499-Base Sequence,
pubmed-meshheading:8675499-Cell Line,
pubmed-meshheading:8675499-Cloning, Molecular,
pubmed-meshheading:8675499-DNA, Complementary,
pubmed-meshheading:8675499-DNA-Binding Proteins,
pubmed-meshheading:8675499-Humans,
pubmed-meshheading:8675499-Molecular Sequence Data,
pubmed-meshheading:8675499-Phosphorylation,
pubmed-meshheading:8675499-RNA Splicing,
pubmed-meshheading:8675499-Repressor Proteins,
pubmed-meshheading:8675499-STAT3 Transcription Factor,
pubmed-meshheading:8675499-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8675499-Trans-Activators,
pubmed-meshheading:8675499-Transcription, Genetic,
pubmed-meshheading:8675499-Tyrosine
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pubmed:year |
1996
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pubmed:articleTitle |
STAT3beta, a splice variant of transcription factor STAT3, is a dominant negative regulator of transcription.
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pubmed:affiliation |
Department of Pulmonary Diseases, University Hospital Utrecht, Utrecht, The Netherlands.
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pubmed:publicationType |
Journal Article
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