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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1996-8-14
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pubmed:databankReference |
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pubmed:abstractText |
We report the expression cloning and characterization of GDNFR-alpha, a novel glycosylphosphatidylinositol-linked cell surface receptor for glial cell line-derived neurotrophic factor (GDNF). GDNFR-alpha binds GDNF specifically and mediates activation of the Ret protein-tyrosine kinase (PTK). Treatment of Neuro-2a cells expressing GDNFR-alpha with GDNF rapidly stimulates Ret autophosphorylation. Ret is also activated by treatment with a combination of GDNF and soluble GDNFR-alpha in cells lacking GDNFR-alpha, and this effect is blocked by a soluble Ret-Fc fusion protein. Ret activation by GDNF was also observed in cultured embryonic rat spinal cord motor neurons, a cell type that responds to GDNF in vivo. A model for the stepwise formation of a GDNF signal-transducing complex including GDNF, GDNFR-alpha, and the Ret PTK is proposed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gdnf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Gdnf protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Cell Line-Derived...,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Cell Line-Derived...,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ret,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ret oncogene protein, Drosophila,
http://linkedlifedata.com/resource/pubmed/chemical/Ret protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ret protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:AltrockB WBW,
pubmed-author:AntonioLL,
pubmed-author:CupplesRR,
pubmed-author:FangMM,
pubmed-author:FoxG MGM,
pubmed-author:HoPP,
pubmed-author:HoYY,
pubmed-author:HolstP LPL,
pubmed-author:JingSS,
pubmed-author:LouisJ CJC,
pubmed-author:LuoYY,
pubmed-author:TamirRR,
pubmed-author:WenDD,
pubmed-author:YoSS
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1113-24
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:8674117-Amino Acid Sequence,
pubmed-meshheading:8674117-Animals,
pubmed-meshheading:8674117-Base Sequence,
pubmed-meshheading:8674117-Cells, Cultured,
pubmed-meshheading:8674117-Cloning, Molecular,
pubmed-meshheading:8674117-Dose-Response Relationship, Drug,
pubmed-meshheading:8674117-Drosophila Proteins,
pubmed-meshheading:8674117-Fetus,
pubmed-meshheading:8674117-Glial Cell Line-Derived Neurotrophic Factor,
pubmed-meshheading:8674117-Glial Cell Line-Derived Neurotrophic Factor Receptors,
pubmed-meshheading:8674117-Glycosylphosphatidylinositols,
pubmed-meshheading:8674117-Membrane Proteins,
pubmed-meshheading:8674117-Mice,
pubmed-meshheading:8674117-Mice, Inbred C57BL,
pubmed-meshheading:8674117-Molecular Sequence Data,
pubmed-meshheading:8674117-Motor Neurons,
pubmed-meshheading:8674117-Nerve Growth Factors,
pubmed-meshheading:8674117-Nerve Tissue Proteins,
pubmed-meshheading:8674117-Phosphorylation,
pubmed-meshheading:8674117-Proto-Oncogene Proteins,
pubmed-meshheading:8674117-Proto-Oncogene Proteins c-ret,
pubmed-meshheading:8674117-Rats,
pubmed-meshheading:8674117-Rats, Sprague-Dawley,
pubmed-meshheading:8674117-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:8674117-Receptors, Nerve Growth Factor,
pubmed-meshheading:8674117-Recombinant Proteins,
pubmed-meshheading:8674117-Retina,
pubmed-meshheading:8674117-Spinal Cord,
pubmed-meshheading:8674117-Tyrosine
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pubmed:year |
1996
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pubmed:articleTitle |
GDNF-induced activation of the ret protein tyrosine kinase is mediated by GDNFR-alpha, a novel receptor for GDNF.
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pubmed:affiliation |
Department of Immunology, Amgen,Inc., Thousand Oaks, California 91320, USA.
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pubmed:publicationType |
Journal Article
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