Linked Life Data

8673249

Source:http://linkedlifedata.com/resource/pubmed/id/8673249

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-8-9
pubmed:abstractText
Amino-acid analysis is a relatively new method for identification of proteins separated by two-dimensional gel electrophoresis and blotted onto polyvinylidene difluoride (PVDF) membranes. This article describes modified amino-acid analysis methods for this purpose. Streamlined sample handling is a key feature of the process. To minimise sample manipulation, a single vial is used for hydrolysis and the protein hydrolysate on PVDF membrane is extracted by a one-step procedure. The hydrolysate should not be stored for long periods before analysis. Applications of the technique are presented to demonstrate the identification procedure. This approach is the most cost-effective and time-effective first step in mass protein screening for a large-scale proteome project.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9673
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
736
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
291-302
pubmed:dateRevised
2009-1-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Large-scale amino-acid analysis for proteome studies.
pubmed:affiliation
Macquarie University Centre for Analytical Biotechnology, Macquarie University, Sydney, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't