Linked Life Data

8671275

Source:http://linkedlifedata.com/resource/pubmed/id/8671275

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-10-21
pubmed:abstractText
In an attempt to enhance embryo development, we have co-cultured 1-cell OF1 mouse embryos on bovine kidney epithelia (Madine-Darby bovine kidney; MDBK) cells in a complex medium called complex mouse tubal fluid (cMTF; based on the energy substrate levels found in the mouse oviduct, containing non-essential amino acids, glutamine and EDTA). To determine the quality of the blastocysts obtained, we examined several parameters: morphology, total cell numbers, inner cell mass (ICM):trophectoderm (TE) ratio, glycolytic activity and viability after transfer. A significantly lower number of blastocysts developed on MDBK cells compared with cMTF medium. cMTF blastocysts had a significantly higher glycolytic activity and a lower blastocyst cell number than those grown in co-culture, while both in-vitro groups had higher ICM:TE ratios compared with in vivo. Blastocysts grown on MDBK cells displayed an elevated ICM number compared with those grown in cMTF medium alone. However, the percentage of fetuses after transfer remained drastically low in both culture groups compared with in-vivo blastocysts. In conclusion, co-culture did not increase the number of zygotes reaching the blastocyst stage. Although co-culture blastocysts show some similarities to in-vivo embryos in cell number and glycolytic activity, no enhancement in viability was observed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0268-1161
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
598-603
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Co-culture of 1-cell outbred mouse embryos on bovine kidney epithelial cells: effect on development, glycolytic activity, inner cell mass:trophectoderm ratios and viability.
pubmed:affiliation
Department of Obstetrics and Gynaecology, Hospital Cantonal University of Geneva, Switzerland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't