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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-8-6
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pubmed:abstractText |
Nucleophosmin/B23 (NPM) is a nucleolar phosphoprotein which shifts from nucleoli to the nucleoplasm in cells treated with certain cytotoxic agents (NPM-translocation). NPM requires GTP for localization into nucleoli (J. Biol. Chem. 268, 5823-5827, 1993). To understand more about NPM's dynamic localization, the effects of lowering ATP on NPM-translocation and rRNA synthesis were studied. When the ATP level in HeLa cells was reduced by sodium azide, NPM-translocation was blocked. Similar results were obtained when ATP was depleted by other agents, suggesting that ATP depletion was responsible for the blocking of NPM-translocation. It was found that newly synthesized rRNA accumulated in the nuclei during ATP-depletion. Significantly larger than normal nucleoli were also observed. These results indicate that NPM may be involved in the transportation of newly synthesized ribosomes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,4-Dinitrophenol,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Alanine,
http://linkedlifedata.com/resource/pubmed/chemical/Antibiotics, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Dinitrophenols,
http://linkedlifedata.com/resource/pubmed/chemical/Mycophenolic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Azide,
http://linkedlifedata.com/resource/pubmed/chemical/alanosine,
http://linkedlifedata.com/resource/pubmed/chemical/nucleophosmin
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
222
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
553-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8670243-2,4-Dinitrophenol,
pubmed-meshheading:8670243-Adenosine Triphosphate,
pubmed-meshheading:8670243-Alanine,
pubmed-meshheading:8670243-Antibiotics, Antineoplastic,
pubmed-meshheading:8670243-Azides,
pubmed-meshheading:8670243-Cell Nucleus,
pubmed-meshheading:8670243-Dinitrophenols,
pubmed-meshheading:8670243-HeLa Cells,
pubmed-meshheading:8670243-Humans,
pubmed-meshheading:8670243-Kinetics,
pubmed-meshheading:8670243-Mycophenolic Acid,
pubmed-meshheading:8670243-Nuclear Proteins,
pubmed-meshheading:8670243-Nucleolus Organizer Region,
pubmed-meshheading:8670243-RNA, Ribosomal,
pubmed-meshheading:8670243-Ribosomes,
pubmed-meshheading:8670243-Sodium Azide
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pubmed:year |
1996
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pubmed:articleTitle |
ATP depletion affects NPM translocation and exportation of rRNA from nuclei.
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pubmed:affiliation |
Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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