|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0006104,
umls-concept:C0006556,
umls-concept:C0009015,
umls-concept:C0017262,
umls-concept:C0185117,
umls-concept:C0382336,
umls-concept:C0679058,
umls-concept:C1522424,
umls-concept:C1547699,
umls-concept:C1709634,
umls-concept:C2700640,
umls-concept:C2911684
|
|
pubmed:dateCreated |
1996-8-5
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
By using a reverse transcription-PCR approach we have cloned a peptide precursor from mouse brain which contains the sequence of orphanin FQ/nociceptin. The mouse sequence of orphanin FQ/nociceptin is identical at the amino acid level with that isolated from rat and porcine brain. Northern analysis of the mRNA encoding the precursor reveals a single band of approx. 1 kb, with the highest levels in the brain and much lower levels in kidney and spleen. Southern analysis is consistent with a single gene. The precursor peptide from mouse contains two putative peptides. Upstream from the orphanin FQ/nociceptin is a 41-amino-acid peptide which is almost identical, except for a six-amino-acid insertion, with the corresponding 35-amino-acid peptide predicted from the rat sequence. Interestingly, the mouse contains a triple AEPGAD repeat within this peptide that is not seen in the rat sequence. Downstream from the orphanin FQ/nociceptin sequence is another 17-amino-acid peptide which is identical with that found in the rat.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-1334555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-1335167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7481766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7515530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7566152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7603458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7797625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7798930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-7877452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-8026588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-8034018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-8034019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-8137918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8670093-8163014
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
315 ( Pt 1)
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
11-3
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:8670093-Amino Acid Sequence,
pubmed-meshheading:8670093-Animals,
pubmed-meshheading:8670093-Base Sequence,
pubmed-meshheading:8670093-Brain,
pubmed-meshheading:8670093-Cloning, Molecular,
pubmed-meshheading:8670093-DNA, Complementary,
pubmed-meshheading:8670093-Gene Expression,
pubmed-meshheading:8670093-Mice,
pubmed-meshheading:8670093-Molecular Sequence Data,
pubmed-meshheading:8670093-Opioid Peptides,
pubmed-meshheading:8670093-Polymerase Chain Reaction,
pubmed-meshheading:8670093-Protein Precursors,
pubmed-meshheading:8670093-RNA, Messenger,
pubmed-meshheading:8670093-Rats,
pubmed-meshheading:8670093-Receptors, Opioid,
pubmed-meshheading:8670093-Swine
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor.
|
|
pubmed:affiliation |
Cotzias Laboratory of Neuro-Oncology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
