Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1996-8-8
pubmed:abstractText
Translational regulation has been identified as one of the key steps in chloroplast-encoded gene expression. Genetic and biochemical analysis with Chlamydomonas reinhardtii has implicated nucleus-encoded factors that interact specifically with the 5' untranslated region of chloroplast mRNAs to mediate light-activated translation. F35 is a nuclear mutation in C. reinhardtii that specifically affects translation of the psbA mRNA (encoding D1, a core polypeptide of photosystem II), causing a photosynthetic deficiency in the mutant strain. The F35 mutant has reduced ribosome association of the psbA mRNA as a result of decreased translation initiation. This reduction in ribosome association correlates with a decrease in the stability of the mRNA. Binding activity of the psbA specific protein complex to the 5' untranslated region of the mRNA is diminished in F35 cells, and two members of this binding complex (RB47 and RB55) are reduced compared with the wild type. These data suggest that alteration of members of the psbA mRNA binding complex in F35 cells results in a reduction in psbA mRNA-protein complex formation, thereby causing a decrease in translation initiation of this mRNA.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-1282351, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-1377098, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-16453694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-16453828, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-1721866, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-2460341, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-2661015, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-2663467, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-3279047, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-3533953, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-3556163, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-5277091, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-6210077, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-7798310, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-7893142, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-7914100, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-7987409, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-7992056, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-8035805, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-8187775, http://linkedlifedata.com/resource/pubmed/commentcorrection/8668172-8440249
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3560-6
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Altered mRNA binding activity and decreased translational initiation in a nuclear mutant lacking translation of the chloroplast psbA mRNA.
pubmed:affiliation
Division of Plant Biology, Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.