Linked Life Data

8668127

Source:http://linkedlifedata.com/resource/pubmed/id/8668127

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-8-5
pubmed:abstractText
The Schizosaccharomyces pombe rhp51+ gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and the Saccharomyces cerevisiae RAD51 protein. Levels of rhp51+ mRNA increase following several types of DNA damage or inhibition of DNA synthesis. An rhp51::ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulating rhp51+ expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (for damage-responsive element), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes from S. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility of rhp51+ expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
251
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-75
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8668127-Base Sequence, pubmed-meshheading:8668127-Cloning, Molecular, pubmed-meshheading:8668127-DNA, Fungal, pubmed-meshheading:8668127-DNA Damage, pubmed-meshheading:8668127-DNA Repair, pubmed-meshheading:8668127-DNA-Binding Proteins, pubmed-meshheading:8668127-Fungal Proteins, pubmed-meshheading:8668127-Gene Deletion, pubmed-meshheading:8668127-Gene Expression Regulation, Fungal, pubmed-meshheading:8668127-Molecular Sequence Data, pubmed-meshheading:8668127-Promoter Regions, Genetic, pubmed-meshheading:8668127-Protein Binding, pubmed-meshheading:8668127-Rad51 Recombinase, pubmed-meshheading:8668127-Rec A Recombinases, pubmed-meshheading:8668127-Saccharomyces cerevisiae Proteins, pubmed-meshheading:8668127-Schizosaccharomyces, pubmed-meshheading:8668127-Schizosaccharomyces pombe Proteins
pubmed:year
1996
pubmed:articleTitle
Identification of the DNA damage-responsive elements of the rhp51+ gene, a recA and RAD51 homolog from the fission yeast Schizosaccharomyces pombe.
pubmed:affiliation
Department of Molecular Biology, Seoul National University, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't