Linked Life Data

8666906

Source:http://linkedlifedata.com/resource/pubmed/id/8666906

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-8-5
pubmed:abstractText
The function of murine dendritic epidermal cells (dEC) remains largely speculative, probably because of the lack of a suitable in vivo model, although previous studies suggest that gamma/delta+ dEC may have originally evolved to serve as a self-protection mechanism(s). Our previous study demonstrated that the epidermis of mice that had spontaneously recovered from cutaneous graft-vs-host disease (GVHD) induced by local injection of CD4+ autoreactive T cells contained unexpectedly large numbers of dEC and became resistant to subsequent attempts to induce GVHD in a site-restricted manner, suggesting that the resistance is mediated by dEC. However, because alpha/beta+ dEC as well as gamma/delta+ dEC were greatly increased in number in the epidermis, it was unclear whether gamma/delta+ dEC are indeed responsible for this protection. The availability of this murine model and mice selectively lacking gamma/delta T cells as a result of disruption of the T cell receptor C delta gene segment allowed us to investigate the role of gamma/delta+ dEC. In the epidermis of gamma/delta T cell-deficient mice (delta-/-), a congenital lack of gamma/delta+ dEC was substituted for by alpha/beta+ dEC of either a CD4-8+ or a CD4-8- phenotype. After intradermal injection of the autoreactive T cells, delta-/- mice developed significantly enhanced delayed-type hypersensitivity responses and cutaneous GVHD, which persisted longer than in heterozygous littermate controls (delta+/-). Surprisingly, resistance to the cutaneous GVHD was not induced in the epidermis of delta-/- mice after spontaneous recovery from the GVHD, whereas the "susceptible" epidermis of delta-/+ mice contained large numbers of alpha/beta dEC comparable to those in "resistant" epidermis of delta+/- mice. Injection of day 16 fetal thymocytes from wild-type mice into delta-/- mice resulted in the appearance of donor-type gamma/delta+ dEC in the epidermis, and reconstitution with gamma/delta+ dEC restored the protective immune response of the epidermis against the GVHD to nearly normal levels. These results indicate that gamma/delta+ dEC are responsible for the site-restricted protection against cutaneous GVHD.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-1826696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-1828619, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-1969918, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-1972173, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-1976696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-2437672, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-2847872, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-2978457, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-3049803, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-3102609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-4155153, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-7816142, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-7973709, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-8097753, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-8105480, http://linkedlifedata.com/resource/pubmed/commentcorrection/8666906-8381716
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
183
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1483-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Resistance to cutaneous graft-vs.-host disease is not induced in T cell receptor delta gene-mutant mice.
pubmed:affiliation
Department of Dermatology, Kyorin University School of Medicine, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't