Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1996-8-8
|
| pubmed:databankReference | |
| pubmed:abstractText |
One of the hallmarks of mucosal-host defense is the clearance of inhaled Ags by alveolar macrophages (AM) through interactions of IgA Abs and IgA Fc receptors (Fc alpha R). AM constitutively expressed Fc alpha R at lower levels than freshly isolated and in vitro-differentiated monocytes as determined by immunofluorescence using four anti-Fc alpha R mAb. SDS-PAGE analysis of iodinated cell surface proteins revealed that Fc alpha R on AM has an Mr of 50 to 65 kDa, slightly lower than that on monocytes (55-75 kDa). Treatment of AM Fc alpha R by N-glycanase gave rise to a protein core of 28 KDa, smaller than the 32-kDa backbone of blood monocytes. AM Fc alpha R molecules were unaffected by phosphatidylinositol-phospholipase C treatment. Fc alpha R transcripts were analyzed by reverse transcription-PCR using primers in the 5' and 3' regions of a U937 Fc alpha R cDNA. Three transcripts were amplified, cloned, and sequenced from AM and/or monocyte mRNA, the full length Fc alpha R and two alternatively spliced products corresponding to deletions of 66 and 288 nucleotides in the portion coding for the extracellular domain; they were named Fc alpha R a.1, a.2, and a.3, respectively. These PCR products were transcribed and translated in vitro into three proteins (Mr 32, 30, and 22 kDa, respectively), in which the 32- and 30-kDa species were immunoprecipitated by an anti-Fc alpha R mAb. The predicted size of the protein encoded by the Fc alpha R a.2 transcript without the leader peptide is Mr approximately 27,400, a value that is consistent with the Mr of AM Fc alpha R backbone. These results indicate that AM express at their surfaces a protein product of an alternatively spliced Fc alpha R transcript, the Fc alpha R a.2 isoform, that might have physiologic relevance in IgA-mediated host defense at mucosal sites.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Fc(alpha) receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Fc
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
156
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4442-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8666819-Alternative Splicing,
pubmed-meshheading:8666819-Animals,
pubmed-meshheading:8666819-Antibodies, Monoclonal,
pubmed-meshheading:8666819-Antigens, CD,
pubmed-meshheading:8666819-Base Sequence,
pubmed-meshheading:8666819-DNA, Complementary,
pubmed-meshheading:8666819-DNA Primers,
pubmed-meshheading:8666819-Gene Expression,
pubmed-meshheading:8666819-Humans,
pubmed-meshheading:8666819-Immunity, Mucosal,
pubmed-meshheading:8666819-Macrophages, Alveolar,
pubmed-meshheading:8666819-Mice,
pubmed-meshheading:8666819-Molecular Sequence Data,
pubmed-meshheading:8666819-Monocytes,
pubmed-meshheading:8666819-Polymerase Chain Reaction,
pubmed-meshheading:8666819-Protein Biosynthesis,
pubmed-meshheading:8666819-Receptors, Fc,
pubmed-meshheading:8666819-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8666819-Transcription, Genetic
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Identification of Fc alpha receptor (CD89) isoforms generated by alternative splicing that are differentially expressed between blood monocytes and alveolar macrophages.
|
| pubmed:affiliation |
INSERM U25, Necker Hospital, Paris, France.
|
| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
|