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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1996-8-5
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pubmed:databankReference | |
pubmed:abstractText |
A rice genomic clone (sps1) coding for sucrose phosphate synthase (SPS) was isolated and sequenced. Rice sps1 contains 13 exons and 12 introns, an unusually long 366-bp leader region with a highly organized primary structure and a promoter region with no obvious homology with eukaryotic promoter consensus sequences. Southern blot analysis showed that SPS is encoded by a single-copy gene in the rice genome. Comparison of the rice, maize, potato and spinach SPS deduced amino acid (aa) sequences showed that these enzymes have a well conserved region comprising their first 700 aa, and a variable C-terminal region. Analysis of rice sps1 expression showed that mRNA levels change during leaf development. SPS activity and mRNA were undetectable in roots.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
May
|
pubmed:issn |
0378-1119
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
170
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-22
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8666248-Amino Acid Sequence,
pubmed-meshheading:8666248-Base Sequence,
pubmed-meshheading:8666248-Blotting, Southern,
pubmed-meshheading:8666248-Cloning, Molecular,
pubmed-meshheading:8666248-DNA, Plant,
pubmed-meshheading:8666248-Gene Expression,
pubmed-meshheading:8666248-Genome, Plant,
pubmed-meshheading:8666248-Glucosyltransferases,
pubmed-meshheading:8666248-Molecular Sequence Data,
pubmed-meshheading:8666248-Oryza sativa,
pubmed-meshheading:8666248-Plant Leaves,
pubmed-meshheading:8666248-Sequence Homology, Amino Acid
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pubmed:year |
1996
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pubmed:articleTitle |
Characterization of a rice sucrose-phosphate synthase-encoding gene.
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pubmed:affiliation |
Departamento de Ingeniería Genética de Plantas, Centro de Investigación y Estudios Avanzados del I.P.N., Unidad Irapuato, Guanajuato, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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